Microfluidic Single-Cell Analysis with Affinity Beads

Microfluidic Single-Cell Analysis with Affinity Beads

Werner, Michael; Palankar, Raghavendra; Arm, Loïc; Hovius, Ruud; Vogel, Horst

2015

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Abstract

A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green). © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Details

Title Microfluidic Single-Cell Analysis with Affinity Beads

Author(s) Werner, Michael ; Palankar, Raghavendra ; Arm, Loïc ; Hovius, Ruud ; Vogel, Horst

Published in Small

Pagination 7

Pages 2607-2613

Date 2015

ISSN 1613-6810

Keywords

affinity beads; microfluidics; optical tweezers; photochemical internalization; single-cell analysis

DOI https://doi.org/10.1002/smll.201402650

Other identifier(s) View record in Web of Science

Laboratories LCPPM

Record Appears in Scientific production and competences > SB - School of Basic Sciences > SB Archives > LCPPM - Laboratory of Physical Chemistry of Polymers and Membranes
Peer-reviewed publications
Work produced at EPFL
Journal Articles
Published

Record creation date 2015-01-30