000204738 001__ 204738
000204738 005__ 20190416220320.0
000204738 037__ $$aCONF
000204738 245__ $$aIntegrated biosensors for cell culture monitoring
000204738 269__ $$a2014
000204738 260__ $$c2014
000204738 336__ $$aConference Papers
000204738 520__ $$aBiosensors for endogenous compounds, such as glucose and lactate, are applied to monitor cell cultures. Cells can be cultivated for several purposes, such as understanding and modeling some biological mechanisms, the development of new drugs and therapies, and in the field of regenerative medicine. We have realized a self-contained monitoring system with remote readout. Metabolite detection is based on oxidases immobilized onto carbon nanotubes. We calibrate the system for glucose and lactate detection in phosphate buffer solution. A hw/sw architecture records the signal generated by the biosensor and transmits it to a remote station by means of a Bluetooth module. We have validated two biosensors for metabolic monitoring in culture medium and we detect lactate production in neuroblastoma cells after 72 h of cultivation. The integrated system proposed in the present work opens new opportunities towards the development of novel tools for cell analysis.
000204738 700__ $$0240269$$g167918$$aDe Micheli, Giovanni
000204738 700__ $$0242418$$g176461$$aBoero, Cristina
000204738 700__ $$aOlivo, Jacopo$$g183208$$0242419
000204738 700__ $$aCarrara, sandro
000204738 7112_ $$dApril 25-28, 2014$$cDalian, China$$a4th Annual World Congress of Molecular & Cell Biology (CMBC)
000204738 773__ $$tProceedings of the 4th Annual World Congress of Molecular & Cell Biology (CMBC)
000204738 8564_ $$uhttps://infoscience.epfl.ch/record/204738/files/CMCB.pdf$$zn/a$$s74508$$yn/a
000204738 909C0 $$xU11140$$0252283$$pLSI1
000204738 909CO $$pIC$$ooai:infoscience.tind.io:204738$$qGLOBAL_SET$$pconf$$pSTI
000204738 917Z8 $$x112915
000204738 937__ $$aEPFL-CONF-204738
000204738 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000204738 980__ $$aCONF