000204544 001__ 204544
000204544 005__ 20190317000057.0
000204544 0247_ $$2doi$$a10.1016/j.brainres.2014.11.044
000204544 02470 $$2ISI$$a000351247900005
000204544 037__ $$aARTICLE
000204544 245__ $$aOptogenetic stimulation of the cochlear nucleus using channelrhodopsin-2 evokes activity in the central auditory pathways
000204544 269__ $$a2015
000204544 260__ $$bElsevier$$c2015$$aAmsterdam
000204544 300__ $$a13
000204544 336__ $$aJournal Articles
000204544 520__ $$aOptogenetics has become an important research tool and is being considered as the basis for several neural prostheses. However, few studies have applied optogenetics to the auditory brainstem. This study explored whether optical activation of the cochlear nucleus (CN) elicited responses in neurons in higher centers of the auditory pathway and whether it elicited an evoked response. Viral-mediated gene transfer was used to express channelrhodopsin-2 (ChR2) in the mouse CN. Blue light was delivered via an optical fiber placed near the surface of the infected CN and recordings were made in higher-level centers. Optical stimulation evoked excitatory multiunit spiking activity throughout the tonotopic axis of the central nucleus of the inferior colliculus (IC) and the auditory cortex (Actx). The pattern and magnitude of IC activity elicited by optical stimulation was comparable to that obtained with a 50 dB SPL acoustic click. This broad pattern of activity was consistent with histological confirmation of green fluorescent protein (GFP) label of cell bodies and axons throughout the CN. Increasing pulse rates up to 320 Hz did not significantly affect threshold or bandwidth of the IC responses, but rates higher than 50 Hz resulted in desynchronized activity. Optical stimulation also evoked an auditory brainstem response, which had a simpler waveform than the response to acoustic stimulation. Control cases showed no responses to optical stimulation. These data suggest that optogenetic control of central auditory neurons is feasible, but opsins with faster channel kinetics may be necessary to convey information at rates typical of many auditory signals.
000204544 6531_ $$aChR2
000204544 6531_ $$aInferior colliculus
000204544 6531_ $$aAuditory cortex
000204544 6531_ $$aSynchronization index
000204544 6531_ $$aNeural prosthesis
000204544 700__ $$aDarrow, K.N.
000204544 700__ $$aSlama, M.C.C.
000204544 700__ $$aKozin, E.D.
000204544 700__ $$aOwoc, M.
000204544 700__ $$aHancock, K.
000204544 700__ $$aKempfle, J.
000204544 700__ $$aEdge, A.
000204544 700__ $$0246296$$g208625$$aLacour, S.
000204544 700__ $$aBoyden, E.
000204544 700__ $$aPolley, D.
000204544 700__ $$aBrown, M.C.
000204544 700__ $$aLee, D.J.
000204544 773__ $$j1599$$tBrain Research$$q44-56
000204544 8564_ $$uhttps://infoscience.epfl.ch/record/204544/files/BrainResearch-2014.pdf$$zPublisher's version$$s1790428$$yPublisher's version
000204544 909C0 $$xU12393$$0252424$$pLSBI
000204544 909CO $$qGLOBAL_SET$$pSTI$$ooai:infoscience.tind.io:204544$$particle
000204544 917Z8 $$x208625
000204544 917Z8 $$x148230
000204544 937__ $$aEPFL-ARTICLE-204544
000204544 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000204544 980__ $$aARTICLE