000204394 001__ 204394
000204394 005__ 20180913062929.0
000204394 0247_ $$2doi$$a10.1016/j.jmr.2007.01.010
000204394 022__ $$a1090-7807
000204394 02470 $$2ISI$$a000246494300004
000204394 037__ $$aARTICLE
000204394 245__ $$aThe role of N-15 CSA and CSA/dipole cross-correlation in N-15 relaxation in solid proteins
000204394 260__ $$bACADEMIC PRESS INC ELSEVIER SCIENCE$$c2007
000204394 269__ $$a2007
000204394 336__ $$aJournal Articles
000204394 520__ $$aThe influence of the N-15 CSA on N-15 longitudinal relaxation is investigated for an amide group in solid proteins in powder form under MAS. This contribution is determined to be typically 20-33%, of the overall longitudinal relaxation rate, at 11.74 and 16.45 T, respectively. The improved treatment is used to analyze the internal dynamics in the protein Crh, in the frame of a motional model of diffusion in a cone, using the explicit average sum approach. Significant variations with respect to the determined dynamics parameters are observed when properly accounting for the contribution of N-15 CSA fluctuations. In general, the fit of experimental data including CSA led to the determination of diffusion times (tau(w)) which are longer than when considering only an N-15-H-1 dipolar relaxation mechanism. CSA-Dipole cross-correlation is shown to play little or no role in protonated solids, in direct contrast to the liquid state case. (C) 2007 Elsevier Inc. All rights reserved.
000204394 6531_ $$anitrogen-15 longitudinal relaxation
000204394 6531_ $$asolid-state NMR
000204394 6531_ $$aprotein dynamics
000204394 6531_ $$across-correlation
000204394 6531_ $$amagic angle spinning
000204394 700__ $$aSein, Julien
000204394 700__ $$aGiraud, Nicolas
000204394 700__ $$aBlackledge, Martin
000204394 700__ $$0248253$$aEmsley, Lyndon$$g251248
000204394 773__ $$j186$$k1$$q26-33$$tJournal of Magnetic Resonance
000204394 909C0 $$0252518$$pLRM$$xU12975
000204394 909CO $$ooai:infoscience.tind.io:204394$$pSB$$particle
000204394 937__ $$aEPFL-ARTICLE-204394
000204394 970__ $$aISI:000246494300004/LRM
000204394 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000204394 980__ $$aARTICLE