000204196 001__ 204196
000204196 005__ 20181203023722.0
000204196 0247_ $$2doi$$a10.1039/c4ra08982k
000204196 022__ $$a2046-2069
000204196 02470 $$2ISI$$a000343711000079
000204196 037__ $$aARTICLE
000204196 245__ $$aProgramming and use of Parylene C fluorescence as a quantitative on-chip reference
000204196 260__ $$aCambridge$$bRoyal Soc Chemistry$$c2014
000204196 269__ $$a2014
000204196 300__ $$a7
000204196 336__ $$aJournal Articles
000204196 520__ $$aA large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parameter variations, like for example non-constant illumination intensity over time. In this study, we exploit Parylene C that is used as a bonding layer material in a microfluidic chip. We first show programming of intermediate Parylene C bonding layer fluorescence (iPBLF) and its characterization as a function of the ultraviolet (UV) dose and Parylene C thickness. This technique requires no additional steps in the fabrication of the microfluidic chip and the fluorescence reference zones can simply be incorporated by local exposure to UV light after fabrication. Next, we demonstrate a fluorescence-based analyte concentration and flow-rate measurement in a microfluidic channel, under changing experimental conditions of illumination intensity and taking different microscope objectives. Sensing is realized by analyzing programmed reference and channel images by straightforward data handling using an open-source image processing tool. We anticipate that the demonstrated method will be a key technique allowing low complexity and reliable quantitative fluorescent measurements, for example in point-of-care and mobile diagnostic applications, where intensity calibration can present a major challenge.
000204196 700__ $$aDupouy, Diego G.
000204196 700__ $$0242804$$aCiftlik, Ata Tuna$$g183749
000204196 700__ $$aTeixidor, Joan
000204196 700__ $$0242801$$aGijs, Martin A. M.$$g113762
000204196 773__ $$j4$$k90$$q49367-49373$$tRsc Advances
000204196 909C0 $$0252094$$pLMIS2$$xU10322
000204196 909CO $$ooai:infoscience.tind.io:204196$$pSTI$$particle
000204196 917Z8 $$x113762
000204196 937__ $$aEPFL-ARTICLE-204196
000204196 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000204196 980__ $$aARTICLE