000204094 001__ 204094
000204094 005__ 20181203023719.0
000204094 0247_ $$2doi$$a10.1016/j.jsb.2014.09.003
000204094 022__ $$a1047-8477
000204094 02470 $$2ISI$$a000344839100008
000204094 037__ $$aARTICLE
000204094 245__ $$aStructural studies suggest a peptidoglycan hydrolase function for the Mycobacterium tuberculosis Tat-secreted protein Rv2525c
000204094 260__ $$aSan Diego$$bElsevier$$c2014
000204094 269__ $$a2014
000204094 300__ $$a9
000204094 336__ $$aJournal Articles
000204094 520__ $$aAmong the few proteins shown to be secreted by the Tat system in Mycobacterium tuberculosis, Rv2525c is of particular interest, since its gene is conserved in the minimal genome of Mycobacterium leprae. Previous evidence linked this protein to cell wall metabolism and sensitivity to beta-lactams. We describe here the crystal structure of Rv2525c that shows a TIM barrel-like fold characteristic of glycoside hydrolases of the GH25 family, which includes prokaryotic and phage-encoded peptidoglycan hydrolases. Structural comparison with other members of this family combined with substrate docking suggest that, although the 'neighbouring group' catalytic mechanism proposed for this family still appears as the most plausible, the identity of residues involved in catalysis in GH25 hydrolases might need to be revised. (C) 2014 Elsevier Inc. All rights reserved.
000204094 6531_ $$aTat secretion pathway
000204094 6531_ $$aGH25
000204094 6531_ $$aPeptidoglycan hydrolase
000204094 6531_ $$aTuberculosis
000204094 700__ $$aBellinzoni, Marco$$uInst Pasteur, Unite Microbiol Struct, F-75724 Paris 15, France
000204094 700__ $$aHaouz, Ahmed$$uInst Pasteur, CNRS, UMR3528, F-75724 Paris 15, France
000204094 700__ $$aMiras, Isabelle$$uInst Pasteur, CNRS, UMR3528, F-75724 Paris 15, France
000204094 700__ $$0244367$$aMagnet, Sophie$$g181734$$uEcole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland
000204094 700__ $$aAndre-Leroux, Gwenaelle$$uInst Pasteur, Unite Microbiol Struct, F-75724 Paris 15, France
000204094 700__ $$0245571$$aMukherjee, Raju$$g212956$$uEcole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland
000204094 700__ $$aShepard, William
000204094 700__ $$0243892$$aCole, Stewart T.$$g177247$$uEcole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland
000204094 700__ $$aAlzari, Pedro M.$$uInst Pasteur, Unite Microbiol Struct, F-75724 Paris 15, France
000204094 773__ $$j188$$k2$$q156-164$$tJournal Of Structural Biology
000204094 909C0 $$0252302$$pUPCOL$$xU11742
000204094 909CO $$ooai:infoscience.tind.io:204094$$pSV$$particle
000204094 917Z8 $$x217823
000204094 937__ $$aEPFL-ARTICLE-204094
000204094 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000204094 980__ $$aARTICLE