000204009 001__ 204009
000204009 005__ 20180913062908.0
000204009 0247_ $$2doi$$a10.1002/bit.25320
000204009 022__ $$a0006-3592
000204009 02470 $$2ISI$$a000344388600007
000204009 037__ $$aARTICLE
000204009 245__ $$aMultilevel Correlations in the Biological Phosphorus Removal Process: from Bacterial Enrichment to Conductivity-Based Metabolic Batch Tests and Polyphosphatase Assays
000204009 260__ $$aHoboken$$bWiley-Blackwell$$c2014
000204009 269__ $$a2014
000204009 300__ $$a15
000204009 336__ $$aJournal Articles
000204009 520__ $$aEnhanced biological phosphorus removal (EBPR) from wastewater relies on the preferential selection of active polyphosphate-accumulating organisms (PAO) in the underlying bacterial community continuum. Efficient management of the bacterial resource requires understanding of population dynamics as well as availability of bioanalytical methods for rapid and regular assessment of relative abundances of active PAOs and their glycogen-accumulating competitors (GAO). A systems approach was adopted here toward the investigation of multilevel correlations from the EBPR bioprocess to the bacterial community, metabolic, and enzymatic levels. Two anaerobic-aerobic sequencing-batch reactors were operated to enrich activated sludge in PAOs and GAOs affiliating with "Candidati Accumulibacter and Competibacter phosphates", respectively. Bacterial selection was optimized by dynamic control of the organic loading rate and the anaerobic contact time. The distinct core bacteriomes mainly comprised populations related to the classes Betaproteobacteria, Cytophagia, and Chloroflexi in the PAO enrichment and of Gammaproteobacteria, Alphaproteobacteria, Acidobacteria, and Sphingobacteria in the GAO enrichment. An anaerobic metabolic batch test based on electrical conductivity evolution and a polyphosphatase enzymatic assay were developed for rapid and low-cost assessment of the active PAO fraction and dephosphatation potential of activated sludge. Linear correlations were obtained between the PAO fraction, biomass specific rate of conductivity increase under anaerobic conditions, and polyphosphate-hydrolyzing activity of PAO/GAO mixtures. The correlations between PAO/GAO ratios, metabolic activities, and conductivity profiles were confirmed by simulations with a mathematical model developed in the aqueous geochemistry software PHREEQC. (C) 2014 Wiley Periodicals, Inc.
000204009 6531_ $$aEBPR
000204009 6531_ $$abacterial community structure
000204009 6531_ $$apolyphosphate-accumulating organisms
000204009 6531_ $$apolyphosphate hydrolysis
000204009 6531_ $$aelectrical conductivity
000204009 6531_ $$aenzymatic activity
000204009 6531_ $$amathematical modelling
000204009 700__ $$0243054$$aWeissbrodt, David G.$$g159151$$uEcole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Environm Biotechnol, CH-1015 Lausanne, Switzerland
000204009 700__ $$0241604$$aMaillard, Julien$$g138380$$uEcole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Environm Biotechnol, CH-1015 Lausanne, Switzerland
000204009 700__ $$0240283$$aBrovelli, Alessandro$$g175968$$u	Ecole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Ecological Engineering , CH-1015 Lausanne, Switzerland
000204009 700__ $$aChabrelie, Alexandre$$uEcole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Environm Biotechnol, CH-1015 Lausanne, Switzerland
000204009 700__ $$aMay, Jonathan$$uEcole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Environm Biotechnol, CH-1015 Lausanne, Switzerland
000204009 700__ $$0240405$$aHolliger, Christof$$g121849$$uEcole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Environm Biotechnol, CH-1015 Lausanne, Switzerland
000204009 773__ $$j111$$k12$$q2421-2435$$tBiotechnology and Bioengineering
000204009 909C0 $$0252125$$pLBE$$xU10268
000204009 909C0 $$0252101$$pECOL$$xU11221
000204009 909CO $$ooai:infoscience.tind.io:204009$$particle$$pENAC
000204009 917Z8 $$x121849
000204009 917Z8 $$x169209
000204009 937__ $$aEPFL-ARTICLE-204009
000204009 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000204009 980__ $$aARTICLE