000203995 001__ 203995
000203995 005__ 20181203023716.0
000203995 0247_ $$2doi$$a10.1002/jmor.20307
000203995 022__ $$a0362-2525
000203995 02470 $$2ISI$$a000345206300003
000203995 037__ $$aARTICLE
000203995 245__ $$aBiomineralization in Newly Settled Recruits of the Scleractinian Coral Pocillopora damicornis
000203995 260__ $$aHoboken$$bWiley-Blackwell$$c2014
000203995 269__ $$a2014
000203995 300__ $$a17
000203995 336__ $$aJournal Articles
000203995 520__ $$aCalcium carbonate biomineralization of scleractinian coral recruits is fundamental to the construction of reefs and their survival under stress from global and local environmental change. Establishing a baseline for how normal, healthy coral recruits initiate skeletal formation is, therefore, warranted. Here, we present a thorough, multiscale, microscopic and spectroscopic investigation of skeletal elements deposited by Pocillopora damicornis recruits, from 12 h to 22 days after settlement in aquarium on a flat substrate. Six growth stages are defined, primarily based on appearance and morphology of successively deposited skeletal structures, with the following average formation time-scales: A (<24 h), B (24-36 h), C (36-48 h), D (48-72 h), E (72-96 h), and F (>10 days). Raman and energy dispersive X-ray spectroscopy indicate the presence of calcite among the earliest components of the basal plate, which consist of micrometer-sized, rod-shaped crystals with rhomboidal habit. All later CaCO3 skeletal structures are composed exclusively of aragonite. High-resolution scanning electron microscopy reveals that, externally, all CaCO3 deposits consist of <100 nm granular units. Fusiform, dumbbell-like, and semispherulitic structures, 25-35 mu m in longest dimension, occur only during the earliest stages (Stages A-C), with morphologies similar to structures formed abiotically or induced by organics in in vitro carbonate crystallization experiments. All other skeletal structures of the basal plate are composed of vertically extending lamellar bundles of granules. From Stage D, straight fibrils, 40-45 nm in width and presumably of organic composition, form bridges between these aragonitic bundles emerging from the growing front of fusing skeletal structures. Our results show a clear evolution in the coral polyp biomineralization process as the carbonate structures develop toward those characterizing the adult skeleton. J. Morphol. 275:1349-1365, 2014. (c) 2014 Wiley Periodicals, Inc.
000203995 6531_ $$acalcium carbonate
000203995 6531_ $$ascanning electron microscopy
000203995 6531_ $$adumbbell
000203995 6531_ $$aRaman spectroscopy
000203995 700__ $$0246345$$aGilis, Melany$$g222061$$uEcole Polytech Fed Lausanne, Lab Biol Geochem, Sch Architecture Civil & Environm Engn ENAC, CH-1015 Lausanne, Switzerland
000203995 700__ $$0246343$$aMeibom, Anders$$g220185$$uEcole Polytech Fed Lausanne, Lab Biol Geochem, Sch Architecture Civil & Environm Engn ENAC, CH-1015 Lausanne, Switzerland
000203995 700__ $$aDomart-Coulon, Isabelle$$uMuseum Natl Hist Nat, CNRS, UMR 7245, Paris, France
000203995 700__ $$aGrauby, Olivier$$uAix Marseille Univ, F-13288 Marseille, France
000203995 700__ $$aStolarski, Jaroslaw
000203995 700__ $$aBaronnet, Alain$$uAix Marseille Univ, F-13288 Marseille, France
000203995 773__ $$j275$$k12$$q1349-1365$$tJournal Of Morphology
000203995 909C0 $$0252426$$pLGB$$xU12552
000203995 909CO $$ooai:infoscience.tind.io:203995$$particle$$pENAC
000203995 917Z8 $$x220185
000203995 937__ $$aEPFL-ARTICLE-203995
000203995 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000203995 980__ $$aARTICLE