000203697 001__ 203697
000203697 005__ 20180913062857.0
000203697 0247_ $$2doi$$a10.1039/C4NR05001K
000203697 022__ $$a2040-3372
000203697 02470 $$2ISI$$a000345486200038
000203697 037__ $$aARTICLE
000203697 245__ $$aProbing the size of proteins with glass nanopores
000203697 260__ $$aCambridge$$bRoyal Soc Chemistry$$c2014
000203697 269__ $$a2014
000203697 300__ $$a8
000203697 336__ $$aJournal Articles
000203697 520__ $$aSingle molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensitivity was enhanced by electron reshaping which decreased the nanopore diameter and created geometries with a reduced sensing length. Further, proteins having molecular weights (MW) ranging from 12 kDa to 480 kDa were detected, which showed that their corresponding current peak amplitude changes according to their MW. In the case of the 12 kDa ComEA protein, its DNA-binding properties to an 800 bp long DNA molecule was investigated. Moreover, the influence of the pH on the charge of the protein was demonstrated by showing a change in the translocation direction. This work emphasizes the wide spectrum of detectable molecules using nanopores from glass nanocapillaries, which stand out because of their inexpensive, lithography-free, and rapid manufacturing process.
000203697 700__ $$aSteinbock, L. J.
000203697 700__ $$aKrishnan, S.
000203697 700__ $$0246637$$aBulushev, R. D.$$g225073
000203697 700__ $$aBorgeaud, S.
000203697 700__ $$0243611$$aBlokesch, M.$$g192269
000203697 700__ $$0244906$$aFeletti, L.$$g192061
000203697 700__ $$0240208$$aRadenovic, A.$$g161458
000203697 773__ $$j6$$k23$$q14380-14387$$tNanoscale
000203697 8564_ $$s2946659$$uhttps://infoscience.epfl.ch/record/203697/files/Nanoscale_Steinbock_2014.pdf$$yn/a$$zn/a
000203697 909C0 $$0252069$$pLBEN$$xU11842
000203697 909C0 $$0252246$$pUPBLO$$xU12076
000203697 909CO $$ooai:infoscience.tind.io:203697$$pSV$$pSTI$$particle
000203697 917Z8 $$x161458
000203697 937__ $$aEPFL-ARTICLE-203697
000203697 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000203697 980__ $$aARTICLE