000202519 001__ 202519
000202519 005__ 20181203023634.0
000202519 0247_ $$2doi$$a10.1039/c4lc00082j
000202519 022__ $$a1473-0197
000202519 02470 $$2ISI$$a000340204300020
000202519 037__ $$aARTICLE
000202519 245__ $$aA droplet-based heterogeneous immunoassay for screening single cells secreting antigen-specific antibodies
000202519 260__ $$aCambridge$$bRoyal Soc Chemistry$$c2014
000202519 269__ $$a2014
000202519 300__ $$a6
000202519 336__ $$aJournal Articles
000202519 520__ $$aHigh throughput heterogeneous immunoassays that screen antigen-specific antibody secreting cells are essential to accelerate monoclonal antibody discovery for therapeutic applications. Here, we introduce a heterogeneous single cell immunoassay based on alginate microparticles as permeable cell culture chambers. Using a microfluidic device, we encapsulated single antibody secreting cells in 35-40 mu m diameter alginate microbeads. We functionalized the alginate to capture the secreted antibodies inside the microparticles, enabling single cell analysis and preventing the crosstalk between the neighboring encapsulated cells. We demonstrated non-covalent functionalization of alginate microparticles by adding three secondary antibodies to the alginate solution to form high molecular weight complexes that become trapped in the porous nanostructure of alginate and capture the secreted antibodies. We screened anti-TNF-alpha antibody-secreting cells from a mixture of antibody-secreting cells.
000202519 700__ $$0242921$$aAkbari, Samin$$g185017$$uHarvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
000202519 700__ $$aPirbodaghi, Tohid$$uHarvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
000202519 773__ $$j14$$k17$$q3275-3280$$tLab On A Chip
000202519 909C0 $$0252447$$pIMT$$xU10343
000202519 909CO $$ooai:infoscience.tind.io:202519$$pSTI$$particle
000202519 937__ $$aEPFL-ARTICLE-202519
000202519 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000202519 980__ $$aARTICLE