000202440 001__ 202440
000202440 005__ 20181203023633.0
000202440 0247_ $$2doi$$a10.1515/bmt-2012-0098
000202440 022__ $$a0013-5585
000202440 02470 $$2ISI$$a000342072900005
000202440 037__ $$aARTICLE
000202440 245__ $$aMultisite monitoring of choline using biosensor microprobe arrays in combination with CMOS circuitry
000202440 260__ $$aBerlin$$bWalter De Gruyter Gmbh$$c2014
000202440 269__ $$a2014
000202440 300__ $$a10
000202440 336__ $$aJournal Articles
000202440 520__ $$aA miniature device enabling parallel in vivo detection of the neurotransmitter choline in multiple brain regions of freely behaving rodents is presented. This is achieved by combining a biosensor microprobe array with a custom-developed CMOS chip. Each silicon microprobe comprises multiple platinum electrodes that are coated with an enzymatic membrane and a permselective layer for selective detection of choline. The biosensors, based on the principle of amperometric detection, exhibit a sensitivity of 157 +/- 35 mu A mM(-1) cm(-2), a limit of detection of below 1 mu M, and a response time in the range of 1 s. With on-chip digitalization and multiplexing, parallel recordings can be performed at a high signal-to-noise ratio with minimal space requirements and with substantial reduction of external signal interference. The layout of the integrated circuitry allows for versatile configuration of the current range and can, therefore, also be used for functionalization of the electrodes before use. The result is a compact, highly integrated system, very convenient for on-site measurements.
000202440 6531_ $$ahead stage
000202440 6531_ $$ain vivo
000202440 6531_ $$amicroelectrode
000202440 6531_ $$amicroelectrode array
000202440 6531_ $$aneurotransmitter
000202440 6531_ $$areal-time monitoring
000202440 700__ $$aFrey, Olivier$$uFed Inst Technol Zurich ETHZ, Dept Biosyst Sci & Engn, Bioengn Lab, CH-4058 Basel, Switzerland
000202440 700__ $$aRothe, Joerg$$uFed Inst Technol Zurich ETHZ, Dept Biosyst Sci & Engn, Bioengn Lab, CH-4058 Basel, Switzerland
000202440 700__ $$aHeer, Flavio$$uFed Inst Technol Zurich ETHZ, Dept Biosyst Sci & Engn, Bioengn Lab, CH-4058 Basel, Switzerland
000202440 700__ $$0243289$$avan der Wal, Peter D.$$g190103
000202440 700__ $$0243301$$ade Rooij, Nico F.$$g104887
000202440 700__ $$aHierlemann, Andreas$$uFed Inst Technol Zurich ETHZ, Dept Biosyst Sci & Engn, Bioengn Lab, CH-4058 Basel, Switzerland
000202440 773__ $$j59$$k4$$q305-314$$tBiomedical Engineering-Biomedizinische Technik
000202440 909C0 $$0252173$$pSAMLAB$$xU10329
000202440 909CO $$ooai:infoscience.tind.io:202440$$particle
000202440 917Z8 $$x190047
000202440 937__ $$aEPFL-ARTICLE-202440
000202440 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000202440 980__ $$aARTICLE
000202440 981__ $$a198797