000202393 001__ 202393
000202393 005__ 20190317000031.0
000202393 0247_ $$2doi$$a10.7554/eLife.03346
000202393 022__ $$a2050-084X
000202393 02470 $$2ISI$$a000341257200001
000202393 037__ $$aARTICLE
000202393 245__ $$aIdentification of the transcription factor ZEB1 as a central component of the adipogenic gene regulatory network
000202393 269__ $$a2014
000202393 260__ $$bElife Sciences Publications Ltd$$c2014$$aCambridge
000202393 300__ $$a65
000202393 336__ $$aJournal Articles
000202393 520__ $$aAdipose tissue is a key determinant of whole body metabolism and energy homeostasis. Unraveling the regulatory mechanisms underlying adipogenesis is therefore highly relevant from a biomedical perspective. Our current understanding of fat cell differentiation is centered on the transcriptional cascades driven by the C/EBP protein family and the master regulator PPAR gamma. To elucidate further components of the adipogenic gene regulatory network, we performed a large-scale transcription factor (TF) screen overexpressing 734 TFs in mouse pre-adipocytes and probed their effect on differentiation. We identified 23 novel pro-adipogenic TFs and characterized the top ranking TF, ZEB1, as being essential for adipogenesis both in vitro and in vivo. Moreover, its expression levels correlate with fat cell differentiation potential in humans. Genomic profiling further revealed that this TF directly targets and controls the expression of most early and late adipogenic regulators, identifying ZEB1 as a central transcriptional component of fat cell differentiation.
000202393 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aGubelmann, Carine
000202393 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aSchwalie, Petra C.
000202393 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aRaghav, Sunil K.
000202393 700__ $$uETH, Inst Food Nutr & Hlth, CH-8603 Schwerzenbach, Switzerland$$aRoeder, Eva
000202393 700__ $$uETH, Inst Food Nutr & Hlth, CH-8603 Schwerzenbach, Switzerland$$aDelessa, Tenagne
000202393 700__ $$uETH, Inst Food Nutr & Hlth, CH-8603 Schwerzenbach, Switzerland$$aKiehlmann, Elke
000202393 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aWaszak, Sebastian M.
000202393 700__ $$0242332$$g177011$$uEcole Polytech Fed Lausanne, Lab Virol & Genet, Global Hlth Inst, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aCorsinotti, Andrea
000202393 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aUdin, Gilles
000202393 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aHolcombe, Wiebke
000202393 700__ $$aRudofsky, Gottfried
000202393 700__ $$0240083$$g167919$$uEcole Polytech Fed Lausanne, Lab Virol & Genet, Global Hlth Inst, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aTrono, Didier
000202393 700__ $$uETH, Inst Food Nutr & Hlth, CH-8603 Schwerzenbach, Switzerland$$aWolfrum, Christian
000202393 700__ $$aDeplancke, Bart$$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland
000202393 773__ $$j3$$tElife$$qe03346
000202393 8564_ $$uhttps://infoscience.epfl.ch/record/202393/files/e03346.full.pdf$$zPublisher's version$$s3394631$$yPublisher's version
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000202393 937__ $$aEPFL-ARTICLE-202393
000202393 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000202393 980__ $$aARTICLE