000202133 001__ 202133
000202133 005__ 20180913062730.0
000202133 0247_ $$2doi$$a10.1016/j.nano.2014.09.007
000202133 022__ $$a1549-9634
000202133 02470 $$2ISI$$a000346846500024
000202133 037__ $$aARTICLE
000202133 245__ $$aImaging liver and brain glycogen metabolism at the nanometer scale
000202133 269__ $$a2015
000202133 260__ $$aAmsterdam$$bElsevier Science Bv$$c2015
000202133 300__ $$a7
000202133 336__ $$aJournal Articles
000202133 520__ $$aIn mammals, glycogen synthesis and degradation are dynamic processes regulating blood and cerebral glucose-levels within a well-defined physiological range. Despite the essential role of glycogen in hepatic and cerebral metabolism, its spatiotemporal distribution at the molecular and cellular level is unclear. By correlating electron microscopy and ultra-high resolution ion microprobe (NanoSIMS) imaging of tissue from fasted mice injected with 13C-labeled glucose, we demonstrate that liver glycogenesis initiates in the hepatocyte perinuclear region before spreading toward the cell membrane. In the mouse brain, we observe that 13C is inhomogeneously incorporated into astrocytic glycogen at a rate ~ 25 times slower than in the liver, in agreement with prior bulk studies. This experiment, using temporally resolved, nanometer-scale imaging of glycogen synthesis and degradation, provides greater insight into glucose metabolism in mammalian organs and shows how this technique can be used to explore biochemical pathways in healthy and diseased states.
000202133 6531_ $$aCarbon-13
000202133 6531_ $$aGlucose metabolism
000202133 6531_ $$aNanoSIMS
000202133 6531_ $$aHepatocytes
000202133 6531_ $$aAstrocytes
000202133 700__ $$0245709$$aTakado, Yuhei$$g205910
000202133 700__ $$0240043$$aKnott, Graham$$g159872
000202133 700__ $$aHumbel, Bruno M.
000202133 700__ $$aEscrig, Stéphane
000202133 700__ $$aMasoodi, Mojgan
000202133 700__ $$0246343$$aMeibom, Anders$$g220185
000202133 700__ $$0243721$$aComment, Arnaud$$g100892
000202133 773__ $$j11$$k1$$q239-245$$tNanomedicine: Nanotechnology, Biology and Medicine
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000202133 937__ $$aEPFL-ARTICLE-202133
000202133 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
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