000202124 001__ 202124
000202124 005__ 20180308105702.0
000202124 0247_ $$2doi$$a10.1039/C4PP00202D
000202124 037__ $$aARTICLE
000202124 245__ $$aTemperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitation
000202124 269__ $$a2014
000202124 260__ $$c2014
000202124 336__ $$aJournal Articles
000202124 520__ $$aAdvanced Search
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 Journal cover: Photochemical & Photobiological Sciences
 Photochemical & Photobiological Sciences
 Issue 12, 2014
 A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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 Paper
 Temperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitation
 Jaroslav Varchola,a   Veronika Huntosova,b   Daniel Jancura,ab   Georges Wagnières,c   Pavol Miskovskyab and   Gregor Bánó*ab  
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 Photochem. Photobiol. Sci., 2014,13, 1781-1787
 
 DOI: 10.1039/C4PP00202D
 Received 06 Jun 2014, Accepted 02 Oct 2014
 First published online 06 Oct 2014
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     Abstract
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 Assessment of partial pressure of oxygen (pO2) by luminescence lifetime measurements of ruthenium coordination complexes has been studied intensively during the last few decades. RuPhen (dichlorotris(1,10-phenanthroline) ruthenium(II) hydrate) is a water soluble molecule that has been tested previously for in vivo pO2 detection. In this work we intended to shed light on the production of singlet oxygen by RuPhen. The quantum yield of singlet oxygen production by RuPhen dissolved in 0.9% aqueous NaCl solution (pH = 6) was measured at physiological temperatures (285–310 K) and various concentrations of molecular oxygen. In order to minimize the bleaching of RuPhen, the samples were excited with low power (<2 mW) laser pulses (20 μs long), created by pulsing a cw laser beam with an acousto-optical modulator. We show that, whereas the RuPhen phosphorescence lifetime decreases rapidly with an increase of temperature (keeping the oxygenation level constant), the quantum yield of singlet oxygen production by RuPhen is almost identical in the temperature range of 285–310 K. For air-saturated conditions at 310 K the measured quantum yield is about 0.25. The depopulation rate constants of the RuPhen 3MLCT (metal-to-ligand charge-transfer) state are determined in the absence and in the presence of oxygen. We determined that the excitation energy for the RuPhen 3MLCT→d–d transition is 49 kJ mol−1 in the 0.9% NaCl solution (pH = 6).
000202124 700__ $$aVarchola, Jaroslav
000202124 700__ $$0245761$$aHuntosova, Veronika$$g217880
000202124 700__ $$aJancura, Daniel
000202124 700__ $$0241203$$aWagnieres, Georges$$g106697
000202124 700__ $$aMiskovsky, Pavol
000202124 700__ $$aBano, Gregor
000202124 773__ $$j13$$k12$$q1781-1787$$tPhotochemical & Photobiological Sciences
000202124 909C0 $$0252359$$pGR-VDB
000202124 909C0 $$0252512$$pGPM
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000202124 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000202124 980__ $$aARTICLE