000201799 001__ 201799
000201799 005__ 20181203023619.0
000201799 0247_ $$2doi$$a10.1002/bit.25328
000201799 022__ $$a0006-3592
000201799 037__ $$aARTICLE
000201799 245__ $$aModeling enzymatic hydrolysis of lignocellulosic substrates using fluorescent confocal microscopy II: Pretreated biomass
000201799 260__ $$bWiley-Blackwell$$c2015
000201799 269__ $$a2015
000201799 336__ $$aJournal Articles
000201799 520__ $$aIn this study, we extend imaging and modeling work that was done in Part I of this report for a pure cellulose substrate (filter paper) to more industrially relevant substrates (untreated and pretreated hardwood and switchgrass). Using confocal fluorescence microscopy, we are able to track both the structure of the biomass particle via its autofluorescence, and bound enzyme from a commercial cellulase cocktail supplemented with a small fraction of fluorescently labeled Trichoderma reseii Cel7A. Imaging was performed throughout hydrolysis at temperatures relevant to industrial processing (50°C). Enzyme bound predominantly to areas with low autofluorescence, where structure loss and lignin removal had occurred during pretreatment; this confirms the importance of these processes for successful hydrolysis. The overall shape of both untreated and pretreated hardwood and switchgrass particles showed little change during enzymatic hydrolysis beyond a drop in autofluorescence intensity. The permanence of shape along with a relatively constant bound enzyme signal throughout hydrolysis was similar to observations previously made for filter paper, and was consistent with a modeling geometry of a hollowing out cylinder with widening pores represented as infinite slits. Modeling estimates of available surface areas for pretreated biomass were consistent with previously reported experimental results. © 2014 Wiley Periodicals, Inc.
000201799 700__ $$0248339$$g154408$$aLuterbacher, Jeremy S.
000201799 700__ $$aMoran-Mirabal, Jose M.
000201799 700__ $$aBurkholder, Eric W.
000201799 700__ $$aWalker, Larry P.
000201799 773__ $$j112$$tBiotechnology and Bioengineering$$k1$$q32-42
000201799 909C0 $$xU12907$$0252511$$pLPDC
000201799 909CO $$pSB$$particle$$ooai:infoscience.tind.io:201799
000201799 917Z8 $$x154408
000201799 917Z8 $$x154408
000201799 917Z8 $$x249835
000201799 937__ $$aEPFL-ARTICLE-201799
000201799 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000201799 980__ $$aARTICLE