000201798 001__ 201798
000201798 005__ 20181203023619.0
000201798 0247_ $$2doi$$a10.1002/bit.25329
000201798 022__ $$a0006-3592
000201798 037__ $$aARTICLE
000201798 245__ $$aModeling enzymatic hydrolysis of lignocellulosic substrates using confocal fluorescence microscopy I: Filter paper cellulose
000201798 260__ $$bWiley-Blackwell$$c2015
000201798 269__ $$a2015
000201798 336__ $$aJournal Articles
000201798 520__ $$aEnzymatic hydrolysis is one of the critical steps in depolymerizing lignocellulosic biomass into fermentable sugars for further upgrading into fuels and/or chemicals. However, many studies still rely on empirical trends to optimize enzymatic reactions. An improved understanding of enzymatic hydrolysis could allow research efforts to follow a rational design guided by an appropriate theoretical framework. In this study, we present a method to image cellulosic substrates with complex three-dimensional structure, such as filter paper, undergoing hydrolysis under conditions relevant to industrial saccharification processes (i.e., temperature of 50°C, using commercial cellulolytic cocktails). Fluorescence intensities resulting from confocal images were used to estimate parameters for a diffusion and reaction model. Furthermore, the observation of a relatively constant bound enzyme fluorescence signal throughout hydrolysis supported our modeling assumption regarding the structure of biomass during hydrolysis. The observed behavior suggests that pore evolution can be modeled as widening of infinitely long slits. The resulting model accurately predicts the concentrations of soluble carbohydrates obtained from independent saccharification experiments conducted in bulk, demonstrating its relevance to biomass conversion work. © 2014 Wiley Periodicals, Inc.
000201798 700__ $$0248339$$aLuterbacher, Jeremy S.$$g154408
000201798 700__ $$aMoran-Mirabal, Jose M.
000201798 700__ $$aBurkholder, Eric W.
000201798 700__ $$aWalker, Larry P.
000201798 773__ $$j112$$k1$$q21-31$$tBiotechnology and Bioengineering
000201798 909C0 $$0252511$$pLPDC$$xU12907
000201798 909CO $$ooai:infoscience.tind.io:201798$$pSB$$particle
000201798 917Z8 $$x154408
000201798 917Z8 $$x154408
000201798 917Z8 $$x242569
000201798 917Z8 $$x249835
000201798 937__ $$aEPFL-ARTICLE-201798
000201798 973__ $$aOTHER$$rNON-REVIEWED$$sPUBLISHED
000201798 980__ $$aARTICLE