000201559 001__ 201559
000201559 005__ 20190317000004.0
000201559 0247_ $$2doi$$a10.1038/jcbfm.2014.155
000201559 022__ $$a1559-7016
000201559 02470 $$2ISI$$a000344428800013
000201559 037__ $$aARTICLE
000201559 245__ $$aNon-invasive diagnostic biomarkers for estimating the onset time of permanent cerebral ischemia
000201559 269__ $$a2014
000201559 260__ $$aNew York$$bNature Publishing Group$$c2014
000201559 300__ $$a8
000201559 336__ $$aJournal Articles
000201559 520__ $$aThe treatments for ischemic stroke can only be administered in a narrow time-window. However, the ischemia onset time is unknown in ~30% of stroke patients (wake-up strokes). The objective of this study was to determine whether MR spectra of ischemic brains might allow the precise estimation of cerebral ischemia onset time. We modeled ischemic stroke in male ICR-CD1 mice using a permanent middle cerebral artery filament occlusion model with laser Doppler control of the regional cerebral blood flow. Mice were then subjected to repeated MRS measurements of ipsilateral striatum at 14.1[thinsp]T. A striking initial increase in [gamma]-aminobutyric acid (GABA) and no increase in glutamine were observed. A steady decline was observed for taurine (Tau), N-acetyl-aspartate (NAA) and similarly for the sum of NAA+Tau+glutamate that mimicked an exponential function. The estimation of the time of onset of permanent ischemia within 6[thinsp]hours in a blinded experiment with mice showed an accuracy of 33[plusmn]10[thinsp]minutes. A plot of GABA, Tau, and neuronal marker concentrations against the ratio of acetate/NAA allowed precise separation of mice whose ischemia onset lay within arbitrarily chosen time-windows. We conclude that 1H-MRS has the potential to detect the clinically relevant time of onset of ischemic stroke.
000201559 6531_ $$aCIBM-AIT
000201559 700__ $$aBerthet, Carole
000201559 700__ $$0243883$$aXin, Lijing$$g169484
000201559 700__ $$aBuscemi, Lara
000201559 700__ $$aBenakis, Corinne
000201559 700__ $$0243712$$aGruetter, Rolf$$g161735
000201559 700__ $$aHirt, Lorenz
000201559 700__ $$0243741$$aLei, Hongxia$$g170746
000201559 773__ $$j34$$k12$$q1848-1855$$tJournal of Cerebral Blood Flow & Metabolism
000201559 8564_ $$s605024$$uhttps://infoscience.epfl.ch/record/201559/files/Berthet_PermIschemia_JCBFM_2014.pdf$$yPublisher's version$$zPublisher's version
000201559 909C0 $$0252276$$pLIFMET$$xU10984
000201559 909C0 $$0252477$$pCIBM$$xU12623
000201559 909CO $$ooai:infoscience.tind.io:201559$$pSB$$particle$$qGLOBAL_SET
000201559 917Z8 $$x161735
000201559 917Z8 $$x161735
000201559 937__ $$aEPFL-ARTICLE-201559
000201559 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000201559 980__ $$aARTICLE