000201514 001__ 201514
000201514 005__ 20190317000003.0
000201514 0247_ $$2doi$$a10.1128/mBio.01409-14
000201514 022__ $$a2150-7511
000201514 02470 $$2ISI$$a000341588100056
000201514 037__ $$aARTICLE
000201514 245__ $$aDNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally Coupled
000201514 269__ $$a2014
000201514 260__ $$bAmerican Society for Microbiology$$c2014$$aWashington
000201514 300__ $$a12
000201514 336__ $$aJournal Articles
000201514 520__ $$aThe physiological state of natural competence for transformation allows certain bacteria to take up free DNA from the environment and to recombine such newly acquired DNA into their chromosomes. However, even though conserved components that are required to undergo natural transformation have been identified in several naturally competent bacteria, our knowledge of the underlying mechanisms of the DNA uptake process remains very limited. To better understand these mechanisms, we investigated the competence-mediated DNA transport in the naturally transformable pathogen Vibrio cholerae. Previously, we used a cell biology-based approach to experimentally address an existing hypothesis, which suggested the competence protein ComEA plays a role in the DNA uptake process across the outer membrane of Gram-negative bacteria. Here, we extended this knowledge by investigating the dynamics of DNA translocation across both membranes. More precisely, we indirectly visualized the transfer of the external DNA from outside the cell into the periplasm followed by the shuttling of the DNA into the cytoplasm. Based on these data, we conclude that for V. cholerae, the DNA translocation across the outer and inner membranes is spatially but not temporally coupled.
000201514 700__ $$aSeitz, Patrick
000201514 700__ $$g192269$$aBlokesch, Melanie$$0243611
000201514 773__ $$j5$$tmBio$$k4$$qe01409-14
000201514 8564_ $$uhttps://infoscience.epfl.ch/record/201514/files/mBio-2014-Seitz-.pdf$$zPublisher's version$$s1495943$$yPublisher's version
000201514 909C0 $$xU12076$$0252246$$pUPBLO
000201514 909CO $$qGLOBAL_SET$$pSV$$ooai:infoscience.tind.io:201514$$particle
000201514 917Z8 $$x192269
000201514 917Z8 $$x182396
000201514 937__ $$aEPFL-ARTICLE-201514
000201514 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000201514 980__ $$aARTICLE