000201444 001__ 201444
000201444 005__ 20180913062649.0
000201444 0247_ $$2doi$$a10.1371/journal.ppat.1004067
000201444 022__ $$a1553-7366
000201444 02470 $$2ISI$$a000337732300007
000201444 037__ $$aARTICLE
000201444 245__ $$aProphenoloxidase Activation Is Required for Survival to Microbial Infections in Drosophila
000201444 260__ $$aSan Francisco$$bPublic Library of Science$$c2014
000201444 269__ $$a2014
000201444 300__ $$a15
000201444 336__ $$aJournal Articles
000201444 520__ $$aThe melanization reaction is a major immune response in Arthropods and involves the rapid synthesis of melanin at the site of infection and injury. A key enzyme in the melanization process is phenoloxidase (PO), which catalyzes the oxidation of phenols to quinones, which subsequently polymerize into melanin. The Drosophila genome encodes three POs, which are primarily produced as zymogens or prophenoloxidases (PPO). Two of them, PPO1 and PPO2, are produced by crystal cells. Here we have generated flies carrying deletions in PPO1 and PPO2. By analyzing these mutations alone and in combination, we clarify the functions of both PPOs in humoral melanization. Our study shows that PPO1 and PPO2 are responsible for all the PO activity in the hemolymph. While PPO1 is involved in the rapid early delivery of PO activity, PPO2 is accumulated in the crystals of crystal cells and provides a storage form that can be deployed in a later phase. Our study also reveals an important role for PPO1 and PPO2 in the survival to infection with Gram-positive bacteria and fungi, underlining the importance of melanization in insect host defense.
000201444 700__ $$0244388$$aBinggeli, Olivier$$g190518$$uEcole Polytech Fed Lausanne, Sch Life Sci, Global Hlth Inst, CH-1015 Lausanne, Switzerland
000201444 700__ $$0244389$$aNeyen, Claudine$$g196305$$uEcole Polytech Fed Lausanne, Sch Life Sci, Global Hlth Inst, CH-1015 Lausanne, Switzerland
000201444 700__ $$aPoidevin, Mickael
000201444 700__ $$0244384$$aLemaitre, Bruno$$g178546$$uEcole Polytech Fed Lausanne, Sch Life Sci, Global Hlth Inst, CH-1015 Lausanne, Switzerland
000201444 773__ $$j10$$k5$$tPlos Pathogens
000201444 909C0 $$0252304$$pUPLEM$$xU11772
000201444 909CO $$ooai:infoscience.tind.io:201444$$pSV$$particle
000201444 917Z8 $$x182725
000201444 937__ $$aEPFL-ARTICLE-201444
000201444 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000201444 980__ $$aARTICLE