000201340 001__ 201340
000201340 005__ 20180913062646.0
000201340 0247_ $$2doi$$a10.1016/j.stemcr.2014.04.010
000201340 022__ $$a2213-6711
000201340 02470 $$2ISI$$a000336653200010
000201340 037__ $$aARTICLE
000201340 245__ $$aDerivation of Traceable and Transplantable Photoreceptors from Mouse Embryonic Stem Cells
000201340 269__ $$a2014
000201340 260__ $$aCambridge$$bCell Press$$c2014
000201340 300__ $$a13
000201340 336__ $$aJournal Articles
000201340 520__ $$aRetinal degenerative diseases resulting in the loss of photoreceptors are one of the major causes of blindness. Photoreceptor replacement therapy is a promising treatment because the transplantation of retina-derived photoreceptors can be applied now to different murine retinopathies to restore visual function. To have an unlimited source of photoreceptors, we derived a transgenic embryonic stem cell (ESC) line in which the Crx-GFP transgene is expressed in photoreceptors and assessed the capacity of a 3D culture protocol to produce integration-competent photoreceptors. This culture system allows the production of a large number of photoreceptors recapitulating the in vivo development. After transplantation, integrated cells showed the typical morphology of mature rods bearing external segments and ribbon synapses. We conclude that a 3D protocol coupled with ESCs provides a safe and renewable source of photoreceptors displaying a development and transplantation competence comparable to photoreceptors from age-matched retinas.
000201340 700__ $$aDecembrini, Sarah$$uUniv Lausanne, Dept Ophthalmol, Jules Gonin Eye Hosp, FAA,Unit Gene Therapy & Stem Cell Biol, CH-1004 Lausanne, Switzerland
000201340 700__ $$0240232$$aKoch, Ute$$g181960$$uEcole Polytech Fed Lausanne, Inst Suisse Rech Expt Canc, CH-1015 Lausanne, Switzerland
000201340 700__ $$0240231$$aRadtke, Freddy$$g172749$$uEcole Polytech Fed Lausanne, Inst Suisse Rech Expt Canc, CH-1015 Lausanne, Switzerland
000201340 700__ $$aMoulin, Alexandre
000201340 700__ $$aArsenijevic, Yvan$$uUniv Lausanne, Dept Ophthalmol, Jules Gonin Eye Hosp, FAA,Unit Gene Therapy & Stem Cell Biol, CH-1004 Lausanne, Switzerland
000201340 773__ $$j2$$k6$$q853-865$$tStem Cell Reports
000201340 8564_ $$s4053050$$uhttps://infoscience.epfl.ch/record/201340/files/Stem%20Cell%20Reports.pdf$$yn/a$$zn/a
000201340 909C0 $$0252086$$pUPRAD$$xU11429
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000201340 917Z8 $$x181933
000201340 937__ $$aEPFL-ARTICLE-201340
000201340 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000201340 980__ $$aARTICLE