Ultra-soft cantilevers and 3-D micro-patterned substrates for contractile bundle tension measurement in living cells

Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far difficult to access in a living cell. Here we propose a microfabricated two-component setup for direct tension measurement on a peripheral bundle within an intact cell. We used 3-D substrates made of silicon elastomer to elevate the cell making the filament bundle at its border accessible from the side, and employed an ultra-soft (spring constant 0.78 nN mu m(-1)) epoxy-based cantilever for mechanical probing. With this setup we were able for the first time to measure the tension in peripheral actin bundles in living primary fibroblasts spread on a rigid substrate.


Published in:
Lab On A Chip, 14, 14, 2539-2547
Year:
2014
Publisher:
Cambridge, Royal Society of Chemistry
ISSN:
1473-0197
Laboratories:




 Record created 2014-08-29, last modified 2018-09-13


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