000200543 001__ 200543
000200543 005__ 20190316235948.0
000200543 0247_ $$2doi$$a10.1016/j.jelechem.2014.08.014
000200543 022__ $$a0022-0728
000200543 02470 $$2ISI$$a000343633700018
000200543 037__ $$aARTICLE
000200543 245__ $$aRapid optimisation of a lactate biosensor design using soft probes scanning electrochemical microscopy
000200543 269__ $$a2014
000200543 260__ $$bElsevier$$c2014$$aLausanne
000200543 300__ $$a7
000200543 336__ $$aJournal Articles
000200543 520__ $$aWe report the mapping of biocatalytically active surfaces, particularly on an express search for optimal immobilization conditions of the enzyme lactate oxidase by means of scanning electrochemical microscopy (SECM). With this aim, soft stylus SECM probes containing a carbon paste ultramicroelectrode were modified with Prussian Blue yielding reproducible hydrogen peroxide (H2O2) sensors with a sensitivity of 1.6 ± 0.5 A·M–1·cm–2 for screening applications. The ultramicroelectrode response was stable under harsh conditions of 1 mM H2O2 during the first hour, while the response decay during the second hour was less than 4 % providing sensor suitability for long-term experiments. SECM imaging in contact mode of different lactate oxidase spots containing membranes allowed for a straightforward optimization of the enzyme immobilization conditions on rough screen-printed carbon paste substrates. The resulting lactate biosensor was characterized by improved analytical performance characteristics: a four times enhanced sensitivity (up to 0.3 A·M–1·cm–2) in comparison to previous reports and a remarkably increased operational stability.
000200543 6531_ $$aScanning electrochemical microscopy
000200543 6531_ $$aPrussian Blue
000200543 6531_ $$aLactate biosensor
000200543 6531_ $$aSoft stylus probes
000200543 700__ $$aPribil, Medeya M.
000200543 700__ $$0242741$$g176551$$aCortes Salazar, Fernando
000200543 700__ $$aAndreyev, Egor A.
000200543 700__ $$0246751$$g208650$$aLesch, Andreas
000200543 700__ $$aKaryakina, Elena E.
000200543 700__ $$aVoronin, Oleg G.
000200543 700__ $$aKaryakin, Arkady A.
000200543 700__ $$aGirault, Hubert H.$$g105258$$0242739
000200543 773__ $$j731$$tJournal of Electroanalytical Chemistry$$q112-118
000200543 8564_ $$uhttps://infoscience.epfl.ch/record/200543/files/1-s2.0-S1572665714003464-main.pdf$$zPublisher's version$$s968417$$yPublisher's version
000200543 8564_ $$uhttps://infoscience.epfl.ch/record/200543/files/mmc1.pdf$$zSupporting Information$$s720744$$ySupporting Information
000200543 909C0 $$xU10100$$0252090$$pLEPA
000200543 909CO $$ooai:infoscience.tind.io:200543$$qGLOBAL_SET$$pSB$$particle
000200543 917Z8 $$x208650
000200543 917Z8 $$x208650
000200543 917Z8 $$x208650
000200543 917Z8 $$x208650
000200543 917Z8 $$x208650
000200543 937__ $$aEPFL-ARTICLE-200543
000200543 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000200543 980__ $$aARTICLE