000200185 001__ 200185
000200185 005__ 20180913062553.0
000200185 037__ $$aPOST_TALK
000200185 245__ $$aA Novel Approach to Design of Metabolic Engineering Strategiesa
000200185 269__ $$a2014
000200185 260__ $$c2014
000200185 336__ $$aPosters
000200185 520__ $$aDynamic nonlinear models of metabolism offer significant advantages as compared to traditional steady-state counterparts for the analysis of metabolic networks and identification of metabolic engineering strategies. Uncertainties in the metabolic network structure, kinetic rate laws, and their corresponding parameters are limiting the development of systematic methodologies for construction of large-scale, dynamic metabolic models. In this study, we proposed a novel methodology for design of metabolic engineering strategies that makes use of these models. We illustrated this methodology using a dynamic model of E. coli composed of 225 reactions and 140 metabolites. Implementation of this methodology and subsequent analysis allowed us to identify globally and locally relevant design strategies to from the current state of metabolic network transition towards a desired state. Using dynamic simulations, we analyzed the network responses upon the chosen perturbations of the network components to explore the feasibility and properties of the identified strategies. The methodology can be used for the design of any metabolic network of interest irrespectively of the organism and/or the physiological conditions.
000200185 6531_ $$ametabolic engineering
000200185 6531_ $$anonlinear models
000200185 6531_ $$aKinetic models
000200185 6531_ $$athermodynamics
000200185 700__ $$0246141$$g221171$$aChakrabarti, Anirikh
000200185 700__ $$0247694$$g241502$$aFengos, Georgios
000200185 700__ $$0245962$$g215631$$aAtaman, Meriç
000200185 700__ $$0243874$$g177860$$aSoh, Keng Cher
000200185 700__ $$aMiskovic, Ljubisa$$g133818$$0240448
000200185 700__ $$aHatzimanikatis, Vassily$$g174688$$0240657
000200185 7112_ $$dJune 15-19, 2014$$cVancouver, Canada$$aMetabolic Engineering X
000200185 909C0 $$xU11422$$0252131$$pLCSB
000200185 909CO $$pSB$$pposter$$ooai:infoscience.tind.io:200185
000200185 917Z8 $$x133818
000200185 937__ $$aEPFL-POSTER-200185
000200185 973__ $$aEPFL
000200185 980__ $$aPOSTER