Fluorogenic probes for live-cell imaging of the cytoskeleton

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.


Published in:
Nature Methods
Year:
2014
Publisher:
London, Nature Publishing Group
ISSN:
1548-7105
Laboratories:




 Record created 2014-06-16, last modified 2018-03-17


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