000199688 001__ 199688
000199688 005__ 20190316235925.0
000199688 0247_ $$2doi$$a10.2217/nnm.12.215
000199688 022__ $$a1743-5889
000199688 02470 $$2ISI$$a000335167200001
000199688 037__ $$aARTICLE
000199688 245__ $$aAssessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy
000199688 260__ $$aLondon$$bFuture Medicine$$c2014
000199688 269__ $$a2014
000199688 300__ $$a14
000199688 336__ $$aJournal Articles
000199688 520__ $$aAim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patients and healthy volunteers, as well as mice models, and Young's modulus stiffness values were determined. Results: Fibers displaying abnormally low mechanical stability were detected in biopsies from patients affected by 11 distinct muscle diseases, and Young's modulus values were commensurate to the severity of the disease. Abnormal myofiber resistance was also observed from consulting patients whose muscle condition could not be detected or unambiguously diagnosed otherwise. Discussion & conclusion: This study provides a proof-of-concept that atomic force microscopy yields a quantitative read-out of human muscle function from clinical biopsies, and that it may thereby complement current muscular dystrophy diagnosis.
000199688 6531_ $$aatomic force microscopy
000199688 6531_ $$amuscular dystrophy
000199688 6531_ $$ananoindentation
000199688 6531_ $$adiagnosis
000199688 700__ $$aZwieten, Van
000199688 700__ $$aRuthger, W.
000199688 700__ $$aPuttini, Stefania$$uCtr Biotechnol UNIL EPFL, Mol Biotechnol Lab, CH-1015 Lausanne, Switzerland
000199688 700__ $$0243924$$aLekka, Malgorzata$$g172733$$uPolish Acad Sci, Henryk Niewodniczanski Inst Nucl Phys, Krakow, Poland
000199688 700__ $$0240685$$aWitz, Guillaume$$g147867$$uCtr Biotechnol UNIL EPFL, Mol Biotechnol Lab, CH-1015 Lausanne, Switzerland
000199688 700__ $$aGicquel-Zouida, Evelyne$$uGenethon, Evry, France
000199688 700__ $$aRichard, Isabelle$$uGenethon, Evry, France
000199688 700__ $$aLobrinus, Johannes A.$$uUniv Lausanne Hosp, Pathol Serv, Lausanne, Switzerland
000199688 700__ $$aChevalley, Francois$$uUniv Lausanne Hosp, Dept Surg, Lausanne, Switzerland
000199688 700__ $$0240039$$aBrune, Harald$$g104651$$uEcole Polytech Fed Lausanne, Lab Nanostruct Surfaces, Lausanne, Switzerland
000199688 700__ $$0240091$$aDietler, Giovanni$$g125942
000199688 700__ $$0240620$$aKulik, Andrzej$$g105590
000199688 700__ $$aKuntzer, Thierry$$uUniv Lausanne Hosp, Dept Clin Neurosci, Nerve Muscle Unit, Lausanne, Switzerland
000199688 700__ $$aMermod, Nicolas$$uCtr Biotechnol UNIL EPFL, Mol Biotechnol Lab, CH-1015 Lausanne, Switzerland
000199688 773__ $$j9$$k4$$q393-406$$tNanomedicine
000199688 8564_ $$s2389240$$uhttps://infoscience.epfl.ch/record/199688/files/Zwieten_14_NMed.pdf$$yn/a$$zPreprint
000199688 909C0 $$0252022$$pLNS$$xU10150
000199688 909C0 $$0252162$$pLPMV$$xU10868
000199688 909CO $$ooai:infoscience.tind.io:199688$$pSB$$particle$$qGLOBAL_SET
000199688 917Z8 $$x219936
000199688 937__ $$aEPFL-ARTICLE-199688
000199688 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000199688 980__ $$aARTICLE