000199539 001__ 199539
000199539 005__ 20180913062529.0
000199539 0247_ $$2doi$$a10.1101/gr.172809.114
000199539 022__ $$a1549-5469
000199539 02470 $$2ISI$$a000339860200001
000199539 037__ $$aARTICLE
000199539 245__ $$aLoss of transcriptional control over endogenous retroelements during reprogramming to pluripotency
000199539 260__ $$aCold Spring Harbor$$bCold Spring Harbor Lab Press, Publications Dept$$c2014
000199539 269__ $$a2014
000199539 300__ $$a9
000199539 336__ $$aJournal Articles
000199539 520__ $$aEndogenous retroelements (EREs) account for about half of the mouse or human genome, and their potential as insertional mutagens and transcriptional perturbators is suppressed by early embryonic epigenetic silencing. Here, we asked how ERE control is maintained during the generation of induced pluripotent stem cells (iPSCs), as this procedure involves profound epigenetic remodeling. We found that all EREs tested were markedly up-regulated during the reprogramming of either mouse embryonic fibroblasts, human CD34(+) cells, or human primary hepatocytes. At the iPSC stage, EREs of some classes were repressed, whereas others remained highly expressed, yielding a pattern somewhat reminiscent of that recorded in embryonic stem cells. However, variability persisted between individual iPSC clones in the control of specific ERE integrants. Both during reprogramming and in iPS cells, the up-regulation of specific EREs significantly impacted on the transcription of nearby cellular genes. While transcription triggered by specific ERE integrants at highly precise developmental stages may be an essential step toward obtaining pluripotent cells, the broad and unspecific unleashing of the repetitive genome observed here may contribute to the inefficiency of the reprogramming process and to the phenotypic heterogeneity of iPSCs.
000199539 6531_ $$aTranscriptome
000199539 700__ $$aFriedli, Marc
000199539 700__ $$0240298$$aTurelli, Priscilla$$g168513
000199539 700__ $$0244067$$aKapopoulou, Adamandia$$g182099
000199539 700__ $$0242341$$aRauwel, Benjamin$$g201005
000199539 700__ $$aCastro-Díaz, Nathaly
000199539 700__ $$aRowe, Helen M
000199539 700__ $$0245536$$aEcco, Gabriela$$g188005
000199539 700__ $$aUnzu, Carmen
000199539 700__ $$aPlanet, Evarist
000199539 700__ $$aLombardo, Angelo
000199539 700__ $$0240300$$aMangeat, Bastien$$g168884
000199539 700__ $$aWildhaber, Barbara E
000199539 700__ $$aNaldini, Luigi
000199539 700__ $$0240083$$aTrono, Didier$$g167919
000199539 773__ $$j24$$k8$$q1251-9$$tGenome research
000199539 909C0 $$0252036$$pLVG$$xU11172
000199539 909CO $$ooai:infoscience.tind.io:199539$$pSV$$particle
000199539 917Z8 $$x169414
000199539 917Z8 $$x169414
000199539 937__ $$aEPFL-ARTICLE-199539
000199539 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000199539 980__ $$aARTICLE