000198830 001__ 198830
000198830 005__ 20190416055541.0
000198830 022__ $$a0022-1767
000198830 037__ $$aARTICLE
000198830 245__ $$aT cell-independent, TLR-induced IL-12p70 production in primary human monocytes
000198830 269__ $$a2006
000198830 260__ $$bAmerican Association of Immunologists$$c2006
000198830 336__ $$aJournal Articles
000198830 520__ $$aIL-12p70 is a key cytokine for the induction of Th1 immune responses. IL-12p70 production in myeloid cells is thought to be strictly controlled by T cell help. In this work we demonstrate that primary human monocytes can produce IL-12p70 in the absence of T cell help. We show that human monocytes express TLR4 and TLR8 but lack TLR3 and TLR7 even after preincubation with type I IFN. Simultaneous stimulation of TLR4 and TLR8 induced IL-12p70 in primary human monocytes. IL-12p70 production in peripheral blood myeloid dendritic cells required combined stimulation of TLR7/8 ligands together with TLR4 or with TLR3 ligands. In the presence of T cell-derived IL-4, but not IFN-gamma, stimulation with TLR7/8 ligands was sufficient to stimulate IL-12p70 production. In monocytes, type I IFN was required but not sufficient to costimulate IL-12p70 induction by TLR8 ligation. Furthermore, TLR8 ligation inhibited LPS-induced IL-10 in monocytes, and LPS alone gained the ability to stimulate IL-12p70 in monocytes when the IL-10 receptor was blocked. Together, these results demonstrate that monocytes are licensed to synthesize IL-12p70 through type I IFN provided via the Toll/IL-1R domain-containing adaptor inducing IFN-beta pathway and the inhibition of IL-10, both provided by combined stimulation with TLR4 and TLR8 ligands, triggering a potent Th1 response before T cell help is established.
000198830 700__ $$aBekeredjian-Ding, Isabelle
000198830 700__ $$aRoth, Susanne Ilona
000198830 700__ $$aGilles, Stefanie
000198830 700__ $$aGiese, Thomas
000198830 700__ $$0248136$$g246669$$aAblasser, Andrea
000198830 700__ $$aHornung, Veit
000198830 700__ $$aEndres, Stefan
000198830 700__ $$aHartmann, Gunther
000198830 773__ $$j176$$tJournal of immunology (Baltimore, Md. : 1950)$$k12$$q7438-46
000198830 8564_ $$uhttps://infoscience.epfl.ch/record/198830/files/J%20Immunol-2006-Bekeredjian-Ding-7438-46.pdf$$zPublisher's version$$s584220$$yPublisher's version
000198830 909C0 $$xU12938$$0252505$$pUPABLASSER
000198830 909CO $$ooai:infoscience.tind.io:198830$$qGLOBAL_SET$$pSV$$particle
000198830 917Z8 $$x182396
000198830 937__ $$aEPFL-ARTICLE-198830
000198830 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000198830 980__ $$aARTICLE