000198770 001__ 198770
000198770 005__ 20180913062511.0
000198770 0247_ $$2doi$$a10.1002/9783527673223.ch3
000198770 020__ $$a978-3-527-33544-2
000198770 037__ $$aBOOK_CHAP
000198770 245__ $$aStructural Analysis of Complex Molecular Systems by High-Resolution and Tandem Mass Spectrometry
000198770 260__ $$aUniversità di Palermo, Dipartimento di Fisica e Chimica$$bWiley-VCH Verlag GmbH & Col. KGaA$$c2014
000198770 269__ $$a2014
000198770 336__ $$aBook Chapters
000198770 520__ $$aIn this chapter, we describe the advances in high-resolution and tandem mass spectrometry applied for structural analysis of complex molecular systems. Focus is made on Fourier transform mass spectrometers (FTMS) because of their ability to routinely provide resolving power (resolution), which is above 100 000, and correspondingly high mass accuracy, which is higher than 2 ppm. The advantages of using these analytical capabilities have been demonstrated for analysis of extremely complex mixtures of molecules of diverse nature and size. What are the recent and envisioned steps in FTMS method and technique development? What are the novel application areas that await these developments? We describe the state-of-the-art in ion cyclotron resonance (ICR) and Orbitrap FTMS and showcase the advantages of FTMS applications for petroleomics and intact protein mass measurements. We briefly overview the current methods of ion activation and dissociation as most widely employed in tandem mass spectrometry (MS/MS) for peptide and protein structure analysis. Finally, we discuss the analytical limitations of the state-of-the-art FTMS and offer a brief introduction to some of the possible solutions to these limitations. Particularly, we describe the achieved and envisioned benefits of advanced signal processing methods aimed specifically at FTMS improvement.
000198770 6531_ $$amass spectrometry
000198770 6531_ $$aFourier transform
000198770 6531_ $$ahigh-resolution MS
000198770 6531_ $$aion cyclotron resonance
000198770 6531_ $$aOrbitrap
000198770 6531_ $$atandem mass spectrometry
000198770 700__ $$0240220$$aTsybin, Yury$$g174689
000198770 720_1 $$aPignataro, Bruno$$eed.
000198770 773__ $$kChapter 3$$q63-90$$tDiscovering the Future of Molecular Sciences
000198770 909C0 $$0252074$$pLSMB$$xU11424
000198770 909CO $$ooai:infoscience.tind.io:198770$$pSB$$pchapter
000198770 917Z8 $$x148577
000198770 937__ $$aEPFL-CHAPTER-198770
000198770 973__ $$aOTHER$$sPUBLISHED
000198770 980__ $$aCHAPTER