@article{Pillai:198631,
      title = {Linking toxicity and adaptive responses across the  transcriptome, proteome, and phenotype of Chlamydomonas  reinhardtii exposed to silver},
      author = {Pillai, Smitha and Behra, Renata and Nestler, Holger and  Suter, Marc J-F and Sigg, Laura and Schirmer, Kristin},
      publisher = {Natl Acad Sciences},
      journal = {Proceedings Of The National Academy Of Sciences Of The  United States Of America},
      address = {Washington},
      number = {9},
      volume = {111},
      pages = {6. 3490-3495},
      year = {2014},
      abstract = {Understanding mechanistic and cellular events underlying a  toxicological outcome allows the prediction of impact of  environmental stressors to organisms living in different  habitats. A systems-based approach aids in characterizing  molecular events, and thereby the cellular pathways that  have been perturbed. However, mapping only adverse outcomes  of a toxicant falls short of describing the stress or  adaptive response that is mounted to maintain homeostasis  on perturbations and may confer resistance to the toxic  insult. Silver is a potential threat to aquatic organisms  because of the increasing use of silver-based  nanomaterials, which release free silver ions. The effects  of silver were investigated at the transcriptome, proteome,  and cellular levels of Chlamydomonas reinhardtii. The cells  instigate a fast transcriptome and proteome response,  including perturbations in copper transport system and  detoxification mechanisms. Silver causes an initial toxic  insult, which leads to a plummeting of ATP and  photosynthesis and damage because of oxidative stress. In  response, the cells mount a defense response to combat  oxidative stress and to eliminate silver via efflux  transporters. From the analysis of the perturbations of the  cell's functions, we derived a detailed mechanistic  understanding of temporal dynamics of toxicity and adaptive  response pathways for C. reinhardtii exposed to silver.},
      url = {http://infoscience.epfl.ch/record/198631},
      doi = {10.1073/pnas.1319388111},
}