000198619 001__ 198619
000198619 005__ 20181203023503.0
000198619 0247_ $$2doi$$a10.1016/j.ymeth.2013.07.019
000198619 022__ $$a1046-2023
000198619 02470 $$2ISI$$a000333773100014
000198619 037__ $$aARTICLE
000198619 245__ $$aRevealing molecular structure and orientation with Stokes vector resolved second harmonic generation microscopy
000198619 269__ $$a2014
000198619 260__ $$aSan Diego$$bAcademic Press Inc Elsevier Science$$c2014
000198619 300__ $$a9
000198619 336__ $$aJournal Articles
000198619 520__ $$aWe report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter. In this way, the critical polarization parameters can be obtained concurrently without the need of repeated image acquisition. The critical polarization parameters, including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. In turn this allows the molecular structure and orientation of the samples to be determined. Use of Stokes polarimetry is critical in determination of the full polarization state of light, and enables discrimination of material properties not possible with conventional crossed-polarized detection schemes. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of targeted specimens. (C) 2013 Elsevier Inc. All rights reserved.
000198619 6531_ $$aOptical scanning microscopy
000198619 6531_ $$aSecond harmonic generation
000198619 6531_ $$aStokes vector
000198619 6531_ $$aUltrafast laser
000198619 700__ $$aMazumder, Nirmal$$uNatl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan
000198619 700__ $$aHu, Chih-Wei$$uNatl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan
000198619 700__ $$aQiu, Jianjun$$uNatl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan
000198619 700__ $$aForeman, Matthew R.$$uUniv London Imperial Coll Sci Technol & Med, Dept Phys, Blackett Lab, London SW7 2BZ, England
000198619 700__ $$aRomero, Carlos Macias
000198619 700__ $$aToeroek, Peter$$uUniv London Imperial Coll Sci Technol & Med, Dept Phys, Blackett Lab, London SW7 2BZ, England
000198619 700__ $$aKao, Fu-Jen$$uNatl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan
000198619 773__ $$j66$$k2$$q237-245$$tMethods
000198619 909C0 $$0252444$$pIBI
000198619 909CO $$ooai:infoscience.tind.io:198619$$particle$$pSV$$pSTI
000198619 937__ $$aEPFL-ARTICLE-198619
000198619 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000198619 980__ $$aARTICLE