High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.


Published in:
Proceedings Of The National Academy Of Sciences Of The United States Of America, 111, 12, 4566-4571
Year:
2014
Publisher:
Washington, Natl Acad Sciences
ISSN:
0027-8424
Keywords:
Laboratories:




 Record created 2014-05-02, last modified 2018-03-17


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