000198553 001__ 198553
000198553 005__ 20190415234920.0
000198553 020__ $$a978-0-12-800281-0
000198553 0247_ $$2doi$$a10.1016/B978-0-12-800281-0.00007-5
000198553 020__ $$a978-0128002810
000198553 022__ $$a0091-679X
000198553 02470 $$2ISI$$a000333375700007
000198553 037__ $$aBOOK_CHAP
000198553 245__ $$aMicrofluidic Patterning of Protein Gradients on Biomimetic Hydrogel Substrates
000198553 260__ $$bElsevier Academic Press Inc$$c2014$$aSan Diego
000198553 269__ $$a2014
000198553 300__ $$a12
000198553 336__ $$aBook Chapters
000198553 490__ $$aMethods in Cell Biology$$v121
000198553 520__ $$aThis protocol describes a versatile microfluidic method to generate tethered protein gradients of virtually any user-defined shape on biomimetic hydrogel substrates. It can be applied to test, in a microenvironment of physiologically relevant stiffness, how cells respond to graded biomolecular signals, for example to elucidate how morphogen proteins affect stem cell fate. The method is based on the use of microfluidic flow focusing to rapidly capture in a step-wise manner tagged biomolecules via affinity binding on the gel surface. The entire patterning process can be performed in <1 h. We illustrate one application of this method, namely, the spatial control of mouse embryonic stem cell self-renewal in response to gradients of the self-renewal-promoting signal leukemia inhibitory factor.
000198553 700__ $$0244265$$g152270$$aCosson, Steffen
000198553 700__ $$0244264$$g106911$$aLutolf, Matthias P.
000198553 720_1 $$aPiel, M.$$eed.
000198553 720_1 $$aThery, M.$$eed.
000198553 773__ $$j121$$tMicropatterning In Cell Biology, Pt C$$q91-102
000198553 8564_ $$uhttps://infoscience.epfl.ch/record/198553/files/1-s2.0-B9780128002810000075-main.pdf$$zPublisher's version$$s1067222$$yPublisher's version
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000198553 937__ $$aEPFL-CHAPTER-198553
000198553 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000198553 980__ $$aCHAPTER