000197923 001__ 197923
000197923 005__ 20180913062432.0
000197923 037__ $$aSTUDENT
000197923 245__ $$aInactivation of pathogens in urine nitrification reactors
000197923 269__ $$a2014
000197923 260__ $$c2014
000197923 336__ $$aStudent Projects
000197923 520__ $$aThrough nutrient recovery by urine separation, the VUNA project aims to develop an effective sanitation system that simultaneously helps address important issues such as environmental pollution and water scarcity. Refinement of the nutrient recovery process presents several challenges: at a technical level, as nitrifying bacteria are sensitive to several parameters; and from a public health perspective, as nutrient recovery from urine implies exposure to pathogens. As part of this problematic, the present study focused on four main objectives: operating nitrification reactors, characterising both virus and bacteria inactivation, and evaluating different inactivation mechanisms. Indicator organisms (bacteriophages and bacteria) were used as surrogates for human pathogens. In a first set of experiments, the bacteriophage MS2 was spiked continuously over 60 days in a continuous flow nitrification reactor. Nitrification did not affect the bacteriophage concentration within the reactor. The second set of experiments consisted of the operation of small scale batch and semi-batch reactors to test in total three different bacteriophages (X147, MS2, Qbeta) and two bacteria (Salmonella typhimerium, Enterococcus spp.) under varying parameters. Pathogens can be inactivated or affected by different mechanisms. Four possible inactivation mechanisms were further evaluated: 1) effect of biological activity in a nitrification treatment system, 2) effect of the air-water interface in a buffer (PBS) and nitrified urine, 3) effect of the complexity of solution, 4) effect of ambient temperature. Results indicated that nitrification caused bacterial inactivation but did not influence the bacteriophages concentration. Air-water interface did not affect bacteria and showed mixed results for bacteriophages. Ambient temperature was not an inactivating parameter for neither of the groups studied. Finally, the bacteriophages presented signs of resistance, possibly due to a protective effect of the complex solution in the nitrification reactor.
000197923 6531_ $$aVUNA
000197923 6531_ $$anitrification
000197923 6531_ $$anutrient recovery
000197923 6531_ $$avirus
000197923 6531_ $$abacteria
000197923 700__ $$0248059$$aSchertenleib, Ariane$$g175801
000197923 720_2 $$0240649$$aKohn, Tamar$$edir.$$g173999
000197923 8564_ $$s1295295$$uhttps://infoscience.epfl.ch/record/197923/files/Schertenleib_Master_thesis_corr.pdf$$yn/a$$zn/a
000197923 909C0 $$0252156$$pLCE$$xU11718
000197923 909CO $$ooai:infoscience.tind.io:197923$$pENAC
000197923 917Z8 $$x173999
000197923 937__ $$aEPFL-STUDENT-197923
000197923 973__ $$aEPFL
000197923 980__ $$aSTUDENT$$bMASTERS