000197878 001__ 197878
000197878 005__ 20190415234919.0
000197878 0247_ $$2doi$$a10.1002/9780470151808.sc01f03s4
000197878 020__ $$a9780470151808
000197878 037__ $$aBOOK_CHAP
000197878 245__ $$aIsolation of hematopoietic stem cells from mouse embryonic stem cells
000197878 260__ $$bWiley$$c2008$$aHoboken, N.J.
000197878 269__ $$a2008
000197878 336__ $$aBook Chapters
000197878 520__ $$aThis unit describes a protocol for the isolation of cells from murine embryonic stem cells with hematopoietic stem cell activity, defined by the ability to reconstitute, long term, multiple lineages of the hematopoietic system of lethally irradiated mice. The protocol subjects hematopoietic progenitors specified in differentiating embryoid bodies to ectopic HoxB4 expression (delivered via retroviral infection), followed by coculture and expansion on OP9 stromal cells in the presence of hematopoietic cytokines for 10 days. The protocol results in the generation of hundreds of millions of cells that can rescue mice from lethal irradiation. Although little is known about the phenotype and frequency of the actual hematopoietic stem cell-like cell within the population of cells generated by this protocol, the protocol establishes a system in which these cells can be further studied and the results ultimately translated to the human system.
000197878 700__ $$aMcKinney-Freeman, Shannon L.
000197878 700__ $$aNaveiras, Olaia
000197878 700__ $$aDaley, George Q.
000197878 720_1 $$aElefanty, Andrew$$eed.
000197878 773__ $$tCurrent protocols in stem cell biology$$qUnit 1F.3
000197878 8564_ $$uhttps://infoscience.epfl.ch/record/197878/files/sc01f03.pdf$$zPublisher's version$$s250111$$yPublisher's version
000197878 909C0 $$xU12818$$0252501$$pGR-NAVEIRAS
000197878 909CO $$ooai:infoscience.tind.io:197878$$pSV$$pbook$$pchapter$$qGLOBAL_SET
000197878 917Z8 $$x182396
000197878 937__ $$aEPFL-CHAPTER-197878
000197878 973__ $$aEPFL$$sPUBLISHED
000197878 980__ $$aCHAPTER