000197850 001__ 197850
000197850 005__ 20180913062429.0
000197850 0247_ $$2doi$$a10.1038/nature08073
000197850 022__ $$a1476-4687
000197850 037__ $$aARTICLE
000197850 245__ $$aBiomechanical forces promote embryonic haematopoiesis
000197850 269__ $$a2009
000197850 260__ $$c2009
000197850 336__ $$aJournal Articles
000197850 520__ $$aBiomechanical forces are emerging as critical regulators of embryogenesis, particularly in the developing cardiovascular system. After initiation of the heartbeat in vertebrates, cells lining the ventral aspect of the dorsal aorta, the placental vessels, and the umbilical and vitelline arteries initiate expression of the transcription factor Runx1 (refs 3-5), a master regulator of haematopoiesis, and give rise to haematopoietic cells. It remains unknown whether the biomechanical forces imposed on the vascular wall at this developmental stage act as a determinant of haematopoietic potential. Here, using mouse embryonic stem cells differentiated in vitro, we show that fluid shear stress increases the expression of Runx1 in CD41(+)c-Kit(+) haematopoietic progenitor cells, concomitantly augmenting their haematopoietic colony-forming potential. Moreover, we find that shear stress increases haematopoietic colony-forming potential and expression of haematopoietic markers in the para-aortic splanchnopleura/aorta-gonads-mesonephros of mouse embryos and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling, compromises haematopoietic potential in vitro and in vivo. Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development.
000197850 6531_ $$aCell Differentiation
000197850 6531_ $$aStress
000197850 6531_ $$aMechanical
000197850 700__ $$aAdamo, Luigi
000197850 700__ $$aNaveiras, Olaia
000197850 700__ $$aWenzel, Pamela L.
000197850 700__ $$aMcKinney-Freeman, Shannon
000197850 700__ $$aMack, Peter J.
000197850 700__ $$aGracia-Sancho, Jorge
000197850 700__ $$aSuchy-Dicey, Astrid
000197850 700__ $$aYoshimoto, Momoko
000197850 700__ $$aLensch, M. William
000197850 700__ $$aYoder, Mervin C.
000197850 700__ $$aGarcía-Cardeña, Guillermo
000197850 700__ $$aDaley, George Q.
000197850 773__ $$j459$$k7250$$q1131-5$$tNature
000197850 8564_ $$s1262900$$uhttps://infoscience.epfl.ch/record/197850/files/nihms-147881.pdf$$yPostprint$$zPostprint
000197850 909C0 $$0252501$$pGR-NAVEIRAS$$xU12818
000197850 909CO $$ooai:infoscience.tind.io:197850$$pSV$$particle
000197850 917Z8 $$x182396
000197850 937__ $$aEPFL-ARTICLE-197850
000197850 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000197850 980__ $$aARTICLE