000197549 001__ 197549
000197549 005__ 20190518121844.0
000197549 02470 $$a1310.5969$$2ArXiv
000197549 037__ $$aARTICLE
000197549 245__ $$aMultiplane 3D superresolution optical fluctuation imaging
000197549 269__ $$a2013
000197549 260__ $$c2013
000197549 336__ $$aJournal Articles
000197549 500__ $$aarXiv:1310.5969 [physics.optics]
000197549 520__ $$aBy switching fluorophores on and off in either a deterministic or a stochastic manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a conventional widefield microscope. So far, three-dimensional (3D) SOFI has only been demonstrated by sequential imaging of multiple depth positions. Here we introduce a versatile imaging scheme which allows for the simultaneous acquisition of multiple focal planes. Using 3D cross-cumulants, we show that the depth sampling can be increased. Consequently, the simultaneous acquisition of multiple focal planes reduces the acquisition time and hence the photo-bleaching of fluorescent markers. We demonstrate multiplane 3D SOFI by imaging the mitochondria network in fixed C2C12 cells over a total volume of 65x65x3.5µm3 without depth scanning.
000197549 6531_ $$a3D imaging
000197549 6531_ $$asuperresolution
000197549 6531_ $$astochastic switching
000197549 6531_ $$across-cumulants
000197549 6531_ $$amolecular statistics
000197549 700__ $$aGeissbuehler, Stefan
000197549 700__ $$0247084$$g229874$$aSharipov, Azat
000197549 700__ $$0246450$$g166900$$aGodinat, Aurélien
000197549 700__ $$0242971$$g187883$$aBocchio, Noelia
000197549 700__ $$0246452$$g217627$$aDubikovskaya, Elena
000197549 700__ $$g120900$$aLasser, Theo$$0240555
000197549 700__ $$aLeutenegger, Marcel$$0240334$$g113715
000197549 773__ $$tarXiv
000197549 8564_ $$uhttps://infoscience.epfl.ch/record/197549/files/manuscript.pdf$$zn/a$$s6786177$$yn/a
000197549 909C0 $$xU10345$$0252112$$pLOB
000197549 909C0 $$0252473$$pLCBIM$$xU12499
000197549 909CO $$qGLOBAL_SET$$pSB$$pSTI$$particle$$ooai:infoscience.tind.io:197549
000197549 917Z8 $$x160907
000197549 937__ $$aEPFL-ARTICLE-197549
000197549 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000197549 980__ $$aARTICLE