Lipolytic biocatalyst based on recyclable magnetite-polysiloxane nanoparticles

This work presents a novel hydrophobic magnetizable nanosupport able to load and valorize the lipase derived from Candida cylindracea (CCL). Nude magnetite nanoparticles (MP) were coated by covalent binding with an ester-polysiloxane (PS). The chemical composition, dimensions, morphology and magnetic properties of the resulted core-shell nanoparticles (MP-PS-CCL) are analyzed. The amount of immobilized lipase increase when loaded from aqueous solutions of up to 12.8 mg/mL CCL, when a lipolytic activity of 74.76 U/g is achieved. For higher concentrations of the loading solution, the activity of immobilized lipase decreases, probably due to the enzyme steric hindrance. MP-PS-CCL exhibits a good lipolytic activity against 4-nitrophenyl laurate (4-NPL), which allows the kinetic study of lipolysis reaction by measuring the amount of released 4-nitrophenol (4-NP), when working at room temperature, in TRIS buffer (pH 8.2). Even after three months of storage, the product is able to sustain up to 4 reusing cycles. (C) 2014 Elsevier B.V. All rights reserved.


Published in:
Applied Surface Science, 292, 898-905
Year:
2014
Publisher:
Amsterdam, Elsevier Science Bv
ISSN:
0169-4332
Keywords:
Laboratories:




 Record created 2014-03-03, last modified 2018-09-13


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