Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly re- producible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively.


Published in:
Review of Scientific Instruments, 85, 1, 015120
Year:
2014
Publisher:
Melville, Amer Inst Physics
ISSN:
1089-7623
Laboratories:




 Record created 2014-02-18, last modified 2018-03-17


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