000196886 001__ 196886
000196886 005__ 20190316235842.0
000196886 0247_ $$2doi$$a10.1016/j.celrep.2013.10.019
000196886 022__ $$a2211-1247
000196886 02470 $$2ISI$$a000328266000014
000196886 037__ $$aARTICLE
000196886 245__ $$aHijacking Multivesicular Bodies Enables Long-Term and Exosome-Mediated Long-Distance Action of Anthrax Toxin
000196886 260__ $$bElsevier$$c2013$$aCambridge
000196886 269__ $$a2013
000196886 300__ $$a11
000196886 336__ $$aJournal Articles
000196886 520__ $$aAnthrax lethal toxin is a classical AB toxin comprised of two components: protective antigen (PA) and lethal factor (LF). Here, we show that following assembly and endocytosis, PA forms a channel that translocates LF, not only into the cytosol, but also into the lumen of endosomal intraluminal vesicles (ILVs). These ILVs can fuse and release LF into the cytosol, where LF can proteolyze and disable host targets. We find that LF can persist in ILVs for days, fully sheltered from proteolytic degradation, both in vitro and in vivo. During this time, ILV-localized LF can be transmitted to daughter cells upon cell division. In addition, LF-containing ILVs can be delivered to the extracellular medium as exosomes. These can deliver LF to the cytosol of naive cells in a manner that is independent of the typical anthrax toxin receptor-mediated trafficking pathway, while being sheltered from neutralizing extracellular factors of the immune system.
000196886 700__ $$uEcole Polytech Fed Lausanne, Global Hlth Inst, Stn 19, CH-1015 Lausanne, Switzerland$$aAbrami, Laurence
000196886 700__ $$0244599$$g204541$$uEcole Polytech Fed Lausanne, Global Hlth Inst, Stn 19, CH-1015 Lausanne, Switzerland$$aBrandi, Lucia
000196886 700__ $$aMoayeri, Mahtab
000196886 700__ $$uUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA$$aBrown, Michael J.
000196886 700__ $$uUniv Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA$$aKrantz, Bryan A.
000196886 700__ $$aLeppla, Stephen H.
000196886 700__ $$avan der Goot, Gisou$$g171549$$0240085
000196886 773__ $$j5$$tCell Reports$$k4$$q986-996
000196886 8564_ $$uhttps://infoscience.epfl.ch/record/196886/files/1-s2.0-S2211124713006037-main.pdf$$zPublisher's version$$s1622109$$yPublisher's version
000196886 909C0 $$xU11271$$0252037$$pVDG
000196886 909CO $$ooai:infoscience.tind.io:196886$$qGLOBAL_SET$$pSV$$particle
000196886 917Z8 $$x181917
000196886 917Z8 $$x182396
000196886 937__ $$aEPFL-ARTICLE-196886
000196886 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000196886 980__ $$aARTICLE