000196813 001__ 196813
000196813 005__ 20180913062357.0
000196813 0247_ $$2doi$$a10.1021/nn405029j
000196813 022__ $$a1936-0851
000196813 02470 $$2ISI$$a000329137100089
000196813 037__ $$aARTICLE
000196813 245__ $$aDNA Trans location through Low-Noise Glass Nanopores
000196813 260__ $$aWashington$$bAmerican Chemical Society$$c2013
000196813 269__ $$a2013
000196813 300__ $$a8
000196813 336__ $$aJournal Articles
000196813 520__ $$aThe effect of electron irradiation-induced shrinking on glass nanocapillaries with diameters ranging from 75 to 14 nm was analyzed by measuring the conductance characteristics with and without DNA translocation. We have investigated nanocapillary shrinking with a scanning electron microscope from several perspectives to understand the geometry of the shrunken nanocapillary. On the basis of this observation, the conductance was modeled with respect to the nanocapillary diameter, which allowed reproducing the experimental results. We then translocated DNA through the shrunken nanocapillaries and measured higher conductance drops for smaller diameters, reaching 1.7 nS for the 14 nm diameter nanocapillary. A model taking into account the conical shape of the shrunken nanocapillaries also supported this dependence. Next, we calculated the noise in the form of the standard deviation of the ionic conductance (between 0.04 and 0.15 nS) to calculate a signal-to-noise ratio (SNR) and compared it with nanopores embedded in 20 nm thick silicon nitride membranes. This shows that although nanocapillaries have smaller signal amplitudes due to their conical shape, they benefit from a lower noise. The glass nanocapillaries have a good SNR of about 25 compared with the SNR of 15 for smaller sized nanopores in silicon nitride membranes. The ability to use a modified model of nanopores to mimic the block conductance by DNA translocation provides a theoretical framework to support experimental results from translocating polymers such as DNA.
000196813 6531_ $$ananopore
000196813 6531_ $$ananocapillary
000196813 6531_ $$aquartz glass
000196813 6531_ $$aDNA translocation
000196813 6531_ $$aresistive pulse technique
000196813 6531_ $$ascanning electron microscope
000196813 700__ $$aSteinbock, Lorenz J.
000196813 700__ $$0246637$$aBulushev, Roman D.$$g225073
000196813 700__ $$aKrishnan, Swati
000196813 700__ $$0244235$$aRaillon, Camille$$g187675
000196813 700__ $$0240208$$aRadenovic, Aleksandra$$g161458
000196813 773__ $$j7$$k12$$q11255-11262$$tAcs Nano
000196813 8564_ $$s1705211$$uhttps://infoscience.epfl.ch/record/196813/files/ACS_Nano_Steinbock_2013.pdf$$yn/a$$zn/a
000196813 909C0 $$0252069$$pLBEN$$xU11842
000196813 909CO $$ooai:infoscience.tind.io:196813$$pSTI$$particle
000196813 917Z8 $$x161458
000196813 917Z8 $$x161458
000196813 937__ $$aEPFL-ARTICLE-196813
000196813 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000196813 980__ $$aARTICLE