000196517 001__ 196517
000196517 005__ 20190812205739.0
000196517 0247_ $$2doi$$a10.1117/12.198705
000196517 037__ $$aCONF
000196517 245__ $$aFluorescence excitation and emission spectra of ALA-induced protoporphyrin IX in normal and tumoral tissue of the human bladder
000196517 260__ $$c1995
000196517 269__ $$a1995
000196517 336__ $$aConference Papers
000196517 520__ $$aIn vivo spectrofluorometric analysis represents a tool to obtain information about fluorophore distribution in tissue. Based on a Peltier-cooled CCD we designed a fluorescence excitation and emission spectrograph which allows to obtain tissue spectra endoscopically and in a clinical environment. Clinical studies were performed on patients with positive cytology or tumor recurrence in the urinary bladder. Patients received a 50 ml instillation of 3% ALA solution at pH 5.5 during 3 to 4 hours and underwent a normal white light cystoscopic examination together with light induced fluorescence photodetection at 5 to 8 hours after the beginning of the instillation. Local fluorescence measurements with a single fiber were performed before photodetection. These showed fluorescence ratios between tumor and normal tissue of 1.5 to 20 with the strongest ratios for exophytic papillary tumors. Fluorescence excitation between 380 nm and 450 nm revealed that the higher Protoporphyrin IX (PPIX) signal on tumor tissue is accompanied by a decrease of the autofluorescence at the emission wavelength of 500 nm.
000196517 700__ $$aForrer, Martin
000196517 700__ $$aGlanzmann, Th.
000196517 700__ $$aMizeret, J.
000196517 700__ $$aBraichotte, Daniel
000196517 700__ $$0241203$$g106697$$aWagnieres, Georges
000196517 700__ $$0240886$$g106613$$aBergh, Hubert
000196517 700__ $$aJichlinski, P.
000196517 700__ $$aLeisinger, H. J.
000196517 773__ $$j2324$$tProceedings of SPIE - The International Society for Optical Engineering$$q84-88
000196517 909C0 $$pLPAS$$0252376$$xU10271
000196517 909CO $$pconf$$ooai:infoscience.tind.io:196517
000196517 917Z8 $$x171848
000196517 937__ $$aEPFL-CONF-196517
000196517 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000196517 980__ $$aCONF