000196423 001__ 196423
000196423 005__ 20181203023423.0
000196423 0247_ $$2doi$$a10.1016/j.bios.2013.12.063
000196423 022__ $$a0956-5663
000196423 02470 $$2ISI$$a000333781000031
000196423 037__ $$aARTICLE
000196423 245__ $$aAbsorbance enhancement in microplate wells for improved-sensitivity biosensors
000196423 269__ $$a2014
000196423 260__ $$aOxford$$bElsevier$$c2014
000196423 300__ $$a6
000196423 336__ $$aJournal Articles
000196423 520__ $$aA genericopticalbiosensingstrategywasdevelopedthatreliesontheabsorbanceenhancementphenom- enon occurringinamultiplescatteringmatrix.Experimentally,insertsmadeofglass fiber membranewere placedintomicroplatewellsinordertosignificantlylengthenthetrajectoryoftheincidentlightthroughthe sampleandthereforeincreasethecorrespondingabsorbance.Enhancementfactorwascalculatedby comparingtheabsorbancevaluesmeasuredforagivenamountofdyewithandwithouttheabsorbance- enhancinginsertsinthewells.Moreover,thedilutionofdyeinsolutionswithdifferentrefractiveindices(RI) clearlyrevealedthattheenhancementfactorincreasedwiththe ΔRI betweenthemembraneandthe surroundingmedium,reachingamaximumvalue(EF425)whenthemembranesweredried.Onthisbasis, two H2O2-biosensingsystemsweredevelopedbasedonthebiofunctionalizationoftheglass fiberinserts either withcytochrome c or horseradishperoxidase(HRP)andthe analyticalperformancesweresystem- aticallycomparedwiththecorrespondingbioassayinsolution.Theefficiencyoftheabsorbance-enhancement approachwasparticularlyclearinthecaseofthecytochrome c-basedbiosensorwithasensitivitygainof40 folds andwiderdynamicrange.Therefore,thedevelopedstrategyrepresentsapromisingwaytoconvert standardcolorimetricbioassaysintoopticalbiosensorswithimprovedsensitivity.
000196423 6531_ $$aNanophotonics
000196423 6531_ $$aPlasmonics
000196423 700__ $$aSuárez, Guillaume
000196423 700__ $$0240847$$aSantschi, Christian$$g117095
000196423 700__ $$aPlateel, Gregory
000196423 700__ $$0244723$$aMartin, Olivier J. F.$$g159310
000196423 700__ $$aRiediker, Michael
000196423 773__ $$j56$$q198-203$$tBiosensors and Bioelectronics
000196423 8564_ $$s1094784$$uhttps://infoscience.epfl.ch/record/196423/files/2014_Suarez_Absorbance_BiosensBioelectron_198.pdf$$yPublisher's version$$zPublisher's version
000196423 909C0 $$0252353$$pNAM$$xU10373
000196423 909CO $$ooai:infoscience.tind.io:196423$$pSTI$$particle
000196423 917Z8 $$x229344
000196423 937__ $$aEPFL-ARTICLE-196423
000196423 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000196423 980__ $$aARTICLE