000196310 001__ 196310
000196310 005__ 20181203023421.0
000196310 0247_ $$2doi$$a10.1021/cb400819c
000196310 022__ $$a1554-8937
000196310 02470 $$2ISI$$a000333477300004
000196310 037__ $$aARTICLE
000196310 245__ $$aA fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red
000196310 260__ $$aWashington$$bAmer Chemical Soc$$c2014
000196310 269__ $$a2014
000196310 300__ $$a7
000196310 336__ $$aJournal Articles
000196310 520__ $$aA fluorogenic probe for plasma membrane proteins based on the dye Nile Red and SNAP-tag is introduced. It takes advantage of Nile Red, a solvatochromic molecule highly fluorescent in an apolar environment, such as cellular membranes, but almost dark in a polar aqueous environment. The probe possesses a tuned affinity for membranes allowing its Nile Red moiety to insert into the lipid bilayer of the plasma membrane, becoming fluorescent, only after its conjugation to a SNAP-tagged plasma membrane protein. The fluorogenic character of the probe was demonstrated for different SNAP-tag fusion proteins, including the human insulin receptor. This work introduces a new approach for generating a powerful turn-on probe for "no-wash" labeling of plasma membrane proteins with numerous applications in bioimaging.
000196310 700__ $$0246789$$aPrifti, Efthymia$$g222580
000196310 700__ $$0240935$$aReymond, Luc$$g155775
000196310 700__ $$aUmebayashi, Miwa
000196310 700__ $$0240613$$aHovius, Ruud$$g105423
000196310 700__ $$aRiezman, Howard
000196310 700__ $$0240057$$aJohnsson, Kai$$g123155
000196310 773__ $$q140114114742001$$tACS Chemical Biology
000196310 909C0 $$0252027$$pLIP$$xU10102
000196310 909CO $$ooai:infoscience.tind.io:196310$$pSB$$particle
000196310 917Z8 $$x105423
000196310 937__ $$aEPFL-ARTICLE-196310
000196310 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000196310 980__ $$aARTICLE