@article{Prifti:196310,
      title = {A fluorogenic probe for SNAP-tagged plasma membrane  proteins based on the solvatochromic molecule Nile Red},
      author = {Prifti, Efthymia and Reymond, Luc and Umebayashi, Miwa and  Hovius, Ruud and Riezman, Howard and Johnsson, Kai},
      publisher = {Amer Chemical Soc},
      journal = {ACS Chemical Biology},
      address = {Washington},
      pages = {7. 140114114742001},
      year = {2014},
      abstract = {A fluorogenic probe for plasma membrane proteins based on  the dye Nile Red and SNAP-tag is introduced. It takes  advantage of Nile Red, a solvatochromic molecule highly  fluorescent in an apolar environment, such as cellular  membranes, but almost dark in a polar aqueous environment.  The probe possesses a tuned affinity for membranes allowing  its Nile Red moiety to insert into the lipid bilayer of the  plasma membrane, becoming fluorescent, only after its  conjugation to a SNAP-tagged plasma membrane protein. The  fluorogenic character of the probe was demonstrated for  different SNAP-tag fusion proteins, including the human  insulin receptor. This work introduces a new approach for  generating a powerful turn-on probe for "no-wash" labeling  of plasma membrane proteins with numerous applications in  bioimaging.},
      url = {http://infoscience.epfl.ch/record/196310},
      doi = {10.1021/cb400819c},
}