000195575 001__ 195575
000195575 005__ 20181203023411.0
000195575 0247_ $$2doi$$a10.1038/cddis.2013.421
000195575 022__ $$a2041-4889
000195575 02470 $$2ISI$$a000327760500015
000195575 037__ $$aARTICLE
000195575 245__ $$aMeasurement of autophagy flux in the nervous system in vivo
000195575 260__ $$bNature Publishing Group$$c2013$$aLondon
000195575 269__ $$a2013
000195575 300__ $$a11
000195575 336__ $$aJournal Articles
000195575 520__ $$aAccurate methods to measure autophagic activity in vivo in neurons are not available, and most of the studies are based on correlative and static measurements of autophagy markers, leading to conflicting interpretations. Autophagy is an essential homeostatic process involved in the degradation of diverse cellular components including organelles and protein aggregates. Autophagy impairment is emerging as a relevant factor driving neurodegeneration in many diseases. Moreover, strategies to modulate autophagy have been shown to provide protection against neurodegeneration. Here we describe a novel and simple strategy to express an autophagy flux reporter in the nervous system of adult animals by the intraventricular delivery of adeno-associated viruses (AAV) into newborn mice. Using this approach we efficiently expressed a monomeric tandem mCherry-GFP-LC3 construct in neurons of the peripheral and central nervous system, allowing the measurement of autophagy activity in pharmacological and disease settings.
000195575 6531_ $$aAutophagy
000195575 6531_ $$aadeno-associated vector (AAV)
000195575 6531_ $$amicrotubule-associated protein 1 light chain 3 (LC3)
000195575 6531_ $$anervous system
000195575 6531_ $$aautophagy flux
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aCastillo, K.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aValenzuela, V.
000195575 700__ $$uNeuroun Biomed Fdn, Santiago, Chile$$aMatus, S.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aNassif, M.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aOnate, M.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aFuentealba, Y.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aEncina, G.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aIrrazabal, T.
000195575 700__ $$uGenzyme Corp, Dept Mol Biol, Framingham, MA 01701 USA$$aParsons, G.
000195575 700__ $$uNeuroun Biomed Fdn, Santiago, Chile$$aCourt, F. A.
000195575 700__ $$uEcole Polytech Fed Lausanne, Brain Mind Inst, Neurodegenerat Studies Lab, Lausanne, Switzerland$$aSchneider, B. L.
000195575 700__ $$uGenzyme Corp, Dept Mol Biol, Framingham, MA 01701 USA$$aArmentano, D.
000195575 700__ $$uUniv Chile, Fac Med, Inst Biomed Sci, Santiago 7, Chile$$aHetz, C.
000195575 773__ $$j4$$tCell Death & Disease
000195575 909C0 $$xU10457$$0252067$$pLEN
000195575 909CO $$pSV$$particle$$ooai:infoscience.tind.io:195575
000195575 917Z8 $$x150283
000195575 937__ $$aEPFL-ARTICLE-195575
000195575 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000195575 980__ $$aARTICLE