000195194 001__ 195194
000195194 005__ 20190316235822.0
000195194 0247_ $$2doi$$a10.1016/j.cca.2013.12.044
000195194 022__ $$a0009-8981
000195194 02470 $$2ISI$$a000335546100025
000195194 037__ $$aARTICLE
000195194 245__ $$aEnzyme and Acid Deconjugation of Plasma Sulfated Metanephrines
000195194 269__ $$a2014
000195194 260__ $$bElsevier$$c2014$$aAmsterdam
000195194 300__ $$a4
000195194 336__ $$aJournal Articles
000195194 520__ $$aBackground: Total (i.e. free + sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report we compare the yield and efficiency of both methods. Methods: Deconjugation rate of synthetic sulfated metanephrines (normetanephrine (S-NMN), metanephrine (S-MN) and methoxytyramine (S-MT)) spiked in charcoal stripped plasma was determined by boiling perchloric acid and compared to sulfatase treatment. Total plasma metanephrines (MN, NMN and MT) were also determined in patient samples by both methods. Results: Complete deconjugation of sulfated metanephrines is achieved after 30 min incubation with 0.1 M boiling perchloric acid or upon sulfatase treatment. Ten minutes of acid hydrolysis (gold-standard) leads to a 30 % underestimation of metanephrine concentrations. The amount of sulfatase required is analyte-dependent (MT>>NMN>MN) though it must contain at least 0.8 mU/ml of sample. The Deming regression curves comparing acid vs enzyme hydrolysis on patient samples assessed that both methods gave similar unbiased concentrations. Conclusion: Enzyme and acid treatments are equivalent and efficient for removing sulfate from metanephrines as long as the optimal protocol is used for each method. However the gold standard method for acid-hydrolysis at 10 min established more than 20 years ago was not satisfactory regarding hydrolysis of metanephrines in plasma.
000195194 6531_ $$aMetanephrines
000195194 6531_ $$aSulfatase
000195194 6531_ $$aDesulfonation
000195194 700__ $$aGlauser, Melanie
000195194 700__ $$aMetrailler, Michael
000195194 700__ $$0241092$$g148571$$aGerber-Lemaire, Sandrine
000195194 700__ $$aCenteno, Catherine
000195194 700__ $$aSeghezzi, Caroline
000195194 700__ $$aDunand, Marielle
000195194 700__ $$aAbid, Karim
000195194 700__ $$aHerren, Adeline
000195194 700__ $$aGrouzmann, Eric
000195194 773__ $$j430$$tClinica Chimica Acta$$q125-128
000195194 8564_ $$uhttps://infoscience.epfl.ch/record/195194/files/ClinChimActa-2014.pdf$$zn/a$$s366883$$yn/a
000195194 909C0 $$xU12326$$0252341$$pLSPN
000195194 909CO $$ooai:infoscience.tind.io:195194$$qGLOBAL_SET$$pSB$$particle
000195194 917Z8 $$x148571
000195194 917Z8 $$x148571
000195194 937__ $$aEPFL-ARTICLE-195194
000195194 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000195194 980__ $$aARTICLE