NOVELTY - The device has a carrier (1) which is provided with micro-cavity (2) for containing medium in direct contact with the object on which the medium is to be applied. Other carrier is provided with ring to protect the medium from environment when the carrier is applied to patient. The sensor is provided with polymer or hydrogel pouch. The sensor is formed as carbon dioxide or metabolite concentration sensor. USE - Device for application of medium to human tissue or animal body such as eye, gingiva, skin, wound or internal organ (all claimed). Can also be used in scalp and mucuous membrane. ADVANTAGE - The carrier is provided with micro-cavity for containing medium in direct contact with the object on which the medium is to be applied. The carrier can be used in simple and precise way to treat the patient. The modified cells or non-epithelial cells to the ocular surface can be transplanted. Transplantation of cells in suspension to other superficial tissues such as gums, mucuous membranes, skin for hair follicle regeneration can be obtained. The dosable application of substances with or to the grafted cells can be obtained to improve engraftment, proliferation and differenciation of cells allowing the use of cells in suspension. The cell culture on lens or another media can be avoided so as to make transplanting easier and faster. The control of the location and of the quantity of cells applied to the target tissue can be allowed.