000191246 001__ 191246
000191246 005__ 20190316235754.0
000191246 0247_ $$2doi$$a10.1073/pnas.1314702110
000191246 022__ $$a1091-6490
000191246 037__ $$aARTICLE
000191246 245__ $$aThe king cobra genome reveals dynamic gene evolution and adaptation in the snake venom system
000191246 269__ $$a2013
000191246 260__ $$bNational Academy of Sciences$$c2013
000191246 336__ $$aJournal Articles
000191246 500__ $$aVonk, Freek J Casewell, Nicholas R Henkel, Christiaan V Heimberg, Alysha M Jansen, Hans J McCleary, Ryan J R Kerkkamp, Harald M E Vos, Rutger A Guerreiro, Isabel Calvete, Juan J Wuster, Wolfgang Woods, Anthony E Logan, Jessica M Harrison, Robert A Castoe, Todd A de Koning, A P Jason Pollock, David D Yandell, Mark Calderon, Diego Renjifo, Camila Currier, Rachel B Salgado, David Pla, Davinia Sanz, Libia Hyder, Asad S Ribeiro, Jose M C Arntzen, Jan W van den Thillart, Guido E E J M Boetzer, Marten Pirovano, Walter Dirks, Ron P Spaink, Herman P Duboule, Denis McGlinn, Edwina Kini, R Manjunatha Richardson, Michael K Journal article Proceedings of the National Academy of Sciences of the United States of America Proc Natl Acad Sci U S A. 2013 Dec 2.
000191246 520__ $$aSnakes are limbless predators, and many species use venom to help overpower relatively large, agile prey. Snake venoms are complex protein mixtures encoded by several multilocus gene families that function synergistically to cause incapacitation. To examine venom evolution, we sequenced and interrogated the genome of a venomous snake, the king cobra (Ophiophagus hannah), and compared it, together with our unique transcriptome, microRNA, and proteome datasets from this species, with data from other vertebrates. In contrast to the platypus, the only other venomous vertebrate with a sequenced genome, we find that snake toxin genes evolve through several distinct co-option mechanisms and exhibit surprisingly variable levels of gene duplication and directional selection that correlate with their functional importance in prey capture. The enigmatic accessory venom gland shows a very different pattern of toxin gene expression from the main venom gland and seems to have recruited toxin-like lectin genes repeatedly for new nontoxic functions. In addition, tissue-specific microRNA analyses suggested the co-option of core genetic regulatory components of the venom secretory system from a pancreatic origin. Although the king cobra is limbless, we recovered coding sequences for all Hox genes involved in amniote limb development, with the exception of Hoxd12. Our results provide a unique view of the origin and evolution of snake venom and reveal multiple genome-level adaptive responses to natural selection in this complex biological weapon system. More generally, they provide insight into mechanisms of protein evolution under strong selection.
000191246 700__ $$aVonk, F. J.
000191246 700__ $$aCasewell, N. R.
000191246 700__ $$aHenkel, C. V.
000191246 700__ $$aHeimberg, A. M.
000191246 700__ $$aJansen, H. J.
000191246 700__ $$aMccleary, R. J. R.
000191246 700__ $$aKerkkamp, H. M. E.
000191246 700__ $$aVos, R. A.
000191246 700__ $$aGuerreiro, I.
000191246 700__ $$aCalvete, J. J.
000191246 700__ $$aWuster, W.
000191246 700__ $$aWoods, A. E.
000191246 700__ $$aLogan, J. M.
000191246 700__ $$aHarrison, R. A.
000191246 700__ $$aCastoe, T. A.
000191246 700__ $$aDe Koning, A. P. J.
000191246 700__ $$aPollock, D. D.
000191246 700__ $$aYandell, M.
000191246 700__ $$aCalderon, D.
000191246 700__ $$aRenjifo, C.
000191246 700__ $$aCurrier, R. B.
000191246 700__ $$aSalgado, D.
000191246 700__ $$aPla, D.
000191246 700__ $$aSanz, L.
000191246 700__ $$aHyder, A. S.
000191246 700__ $$aRibeiro, J. M. C.
000191246 700__ $$aArntzen, J. W.
000191246 700__ $$aVan Den Thillart, G. E. E. J. M.
000191246 700__ $$aBoetzer, M.
000191246 700__ $$aPirovano, W.
000191246 700__ $$aDirks, R. P.
000191246 700__ $$aSpaink, H. P.
000191246 700__ $$0240276$$aDuboule, D.$$g141236
000191246 700__ $$aMcglinn, E.
000191246 700__ $$aKini, R. M.
000191246 700__ $$aRichardson, M. K.
000191246 773__ $$tProceedings of the National Academy of Sciences
000191246 8564_ $$uhttp://www.ncbi.nlm.nih.gov/pubmed/24297900$$zURL
000191246 8564_ $$s1370391$$uhttps://infoscience.epfl.ch/record/191246/files/PNAS-2013-Vonk-1314702110.pdf$$yn/a$$zn/a
000191246 909C0 $$0252099$$pUPDUB$$xU11705
000191246 909CO $$ooai:infoscience.tind.io:191246$$pSV$$particle$$qGLOBAL_SET
000191246 917Z8 $$x182482
000191246 937__ $$aEPFL-ARTICLE-191246
000191246 973__ $$aEPFL$$rNON-REVIEWED$$sPUBLISHED
000191246 980__ $$aARTICLE