000191186 001__ 191186
000191186 005__ 20190316235753.0
000191186 022__ $$a2041-1723
000191186 0247_ $$2doi$$a10.1038/ncomms3848
000191186 037__ $$aARTICLE
000191186 245__ $$aA quantitative telomeric chromatin isolation protocol identifies different telomeric states
000191186 269__ $$a2013
000191186 260__ $$bNature Publishing Group$$c2013$$aLondon
000191186 300__ $$a12
000191186 336__ $$aJournal Articles
000191186 520__ $$aTelomere composition changes during tumourigenesis, aging and in telomere syndromes in a poorly defined manner. Here we develop a quantitative telomeric chromatin isolation protocol (QTIP) for human cells, in which chromatin is cross-linked, immunopurified and analysed by mass spectrometry. QTIP involves stable isotope labelling by amino acids in cell culture (SILAC) to compare and identify quantitative differences in telomere protein composition of cells from various states. With QTIP, we specifically enrich telomeric DNA and all shelterin components. We validate the method characterizing changes at dysfunctional telomeres, and identify and validate known, as well as novel telomere-associated polypeptides including all THO subunits, SMCHD1 and LRIF1. We apply QTIP to long and short telomeres and detect increased density of SMCHD1 and LRIF1 and increased association of the shelterins TRF1, TIN2, TPP1 and POT1 with long telomeres. Our results validate QTIP to study telomeric states during normal development and in disease
000191186 700__ $$0243158$$g187686$$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aGrolimund, Larissa
000191186 700__ $$0245337$$g212997$$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aAeby, Eric
000191186 700__ $$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aHamelin, Romain
000191186 700__ $$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aArmand, Florence
000191186 700__ $$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aChiappe, Diego
000191186 700__ $$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aMoniatte, Marc
000191186 700__ $$aLingner, Joachim$$uEcole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland$$g168670$$0240570
000191186 773__ $$j4$$tNature Communications$$q2848
000191186 8564_ $$uhttps://infoscience.epfl.ch/record/191186/files/ncomms3848.pdf$$zPublisher's version$$s1253414$$yPublisher's version
000191186 909C0 $$xU11159$$0252147$$pUPLIN
000191186 909CO $$ooai:infoscience.tind.io:191186$$qGLOBAL_SET$$pSV$$particle
000191186 917Z8 $$x168670
000191186 917Z8 $$x182396
000191186 937__ $$aEPFL-ARTICLE-191186
000191186 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000191186 980__ $$aARTICLE