000190682 001__ 190682
000190682 005__ 20180913062145.0
000190682 0247_ $$a10.1117/12.822989$$2doi
000190682 037__ $$aCONF
000190682 245__ $$aIn vitro and in vivo studies of new photoluminescent oxygen sensors for non-invasive intravascular pO 2 measurements
000190682 269__ $$a2009
000190682 260__ $$c2009
000190682 336__ $$aConference Papers
000190682 490__ $$v7380$$aSPIE Proceedings
000190682 520__ $$aThe concentration of oxygen and its rate of consumption are important factors playing a role in PDT and radiotherapy. One of the methods for measuring the tissular oxygen partial pressure (pO 2) is based on the use of luminophores presenting an oxygen-dependent quenching of their phosphorescence. The time-resolved luminescence spectroscopy of palladium (PdTCPP) or ruthenium (RuDPP) porphyrin complexes is used for this purpose. Unfortunately, these porphyrin derivatives are phototoxic and leak rapidly out of the blood vessels, making them unsuitable for measuring tissular and or intravascular pO 2. Therefore, this research aimed at developing and testing new biocompatible, non-phototoxic oxygen sensors based on palladium complexes incorporated into oxygen permeable, polysaccharide-based nanoparticles appropriate for noninvasive in situ and in vivo measurements of the pO 2. In vitro studies, performed with an optical fiber-based time-resolved spectrophotometer, showed that the incorporation of such pO 2 probes in nanovectors reduces their sensitivity to oxygen as well as their photobleaching by less than one order of magnitude. However, in vivo biocompatibility studies performed on the chick's embryo chorioallantoic membrane (CAM) model demonstrated that the luminescence of those oxygen probes tends to be heterogeneously distributed within the vasculature. In addition, these probes induce a 'clumping tendency', resulting in a more or less decreased viability of the embryos. © 2009 Copyright SPIE - The International Society for Optical Engineering.
000190682 6531_ $$aCAM
000190682 6531_ $$achick's chorioallantoic membrane model
000190682 6531_ $$aoxygen
000190682 6531_ $$aPDT
000190682 6531_ $$aPdTCPP
000190682 6531_ $$aphosphorescence quenching
000190682 700__ $$g168504$$aNowak-Sliwinska, P.$$0245027
000190682 700__ $$aForte, E.
000190682 700__ $$g106613$$aVan Den Bergh, H.$$0240886
000190682 700__ $$g106697$$aWagnières, G.$$0241203
000190682 773__ $$q73806N$$tProgress in Biomedical Optics and Imaging - Proceedings of SPIE
000190682 8560_ $$falain.borel@epfl.ch
000190682 909C0 $$xU9$$0252010$$pLCOM
000190682 909C0 $$pGR-VDB$$0252359
000190682 909C0 $$0252512$$pGPM
000190682 909CO $$pconf$$pSTI$$pSB$$ooai:infoscience.tind.io:190682
000190682 917Z8 $$x171848
000190682 937__ $$aEPFL-CONF-190682
000190682 973__ $$rREVIEWED$$aEPFL
000190682 980__ $$aCONF